52. イネにおけるフィトアレキシン生合成に関与するent-, syn-CDP合成酵素遺伝子の異所的発現

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In rice (Oryza sativa), diterpene phytoalexins, such as momilactones, oryzalexins and phytocassanes, are biosynthesized from geranylgeranyl diphosphate (GGDP) via ent-copalyl diphosphate (ent-CDP) or syn-CDP. We isolated OsCyc2 and OsCyc1, which encode ent-CDP synthase and syn-CDP synthase respectively, from rice (cv. Nipponbare). These two genes were suggested to be responsible for phytoalexin biosynthesis. In this report, we introduced OsCyc2 or OsCyc1 into a severe-dwarf rice (K2540), a loss-of-function mutant of OsCPS1 encoding ent-CDP synthase for gibbrellin biosynthesis, and a wild-type rice (Taichung 65, T65) by Agrobacterium-mediated transformation system. All of 9 lines of the transgenic K2540 overexpressing OsCyc2 showed semi-dwarf phenotype, suggesting that overexpression of OsCyc2 recovered the phenotype of K2540 partially. We could not obtain any transgenic K2540 lines overexpressing OsCyc1, because of its unsuccessful complementation. Our results showed that ent-CDP synthesized by OsCyc2 for phytoalexin biosynthesis could partially complement gibberellin-deficient dwarf phenotype. Although 5 lines of transgenic T65 overexpressing OsCyc2 showed dwarf (1 line) or semi-dwarf (4 lines) phenotype, 11 lines overexpressing OsCyc1 exhibited dwarf (4 lines) or semi-dwarf (7 lines) phenotype. These results suggested that overexpression of OsCyc1, syn-CDP synthase, might compete GGDP against OsCPS1, ent-CDP synthase for gibberellin biosynthesis.
2004-10-13

52. イネにおけるフィトアレキシン生合成に関与するent-, syn-CDP合成酵素遺伝子の異所的発現

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