87 レタスのジベレリン生合成酵素遺伝子のプロモーター領域 DNA の単離

概要

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Germination of photoblastic lettuce (Lactuca sativa. L. cv. Grand Rapids) seed is regulated by photochrome. Our previous study showed that lettuce gibbrerllin (GA) 3 β-hydroxylase 1 gene (Ls3h1) is up-regulated by red light irradiation and successively endogenous levels of GA_1 (bioactive GA) was increased in the lettuce seeds, resulting in germination. In order to investigate the mechanism of regulation of Ls3h1 gene expression, we tried to isolate 5' upstream region of the genomic DNA containing promoter. By PCR using genome walker kit, about 3kb promoter region was successfully isolated. Promoter of Ls20ox2 gene, down-regulated by red light irradiation, was also isolated by inverse PCR method. We will compare the sequences of both genes from Grand Rapids with those from non-photoblastic (cv.Santanasu), in which Ls3h1 gene expressed even in the dark.
植物化学調節学会の論文
2002-10-31