53. ヤマノイモのアブシジン酸8'位水酸化酵素遺伝子のクローニング

概要

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In contrast to the case of dormancy of many plants, dormancy of bulbils of Dioscorea japonica is induced by gibberellins (GAs) as well as abscisic acid (ABA), which is referred to as GA-induced dormancy. In a series of the researches on the cross-talk between GA and ABA in the GA-induced dormancy of Dioscorea, ABA has been proved to be metabolized not only to dihydrophaseic acid through 8'-hydroxyABAbut also to 7'-hydroxyABA. We tried to isolate cDNA encoding ABA8'-hydroxylase from a whole juvenile sprout from the bulbil of D. japonica, and consequently succeeded in cloning two full length homologs of the gene (DjABA8'ox1 and DjABA8'ox2). We are to plan to analyze enzyme function of the product proteins translated from, and expression of the transcripts of these two homologs in relation to dormancy regulation in the bulbils of D. japonica.
植物化学調節学会の論文
2005-10-13