42.ヤマノイモのアブシジン酸代謝酵素遺伝子の発現解析(口頭発表)

概要

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In contrast to the case of dormancy of many plants, dormancy of bulbils of D. japonica is induced by gibberellins (GAs) as well as abscisic acid (ABA), which is referred to as GA-induced dormancy. It has been proved that ABA is, in bulbils of D. japonica, metabolized not only to dihydrophaseic acid through 8' -hydroxyABA but also to 7'-hydroxyABA. Along the line of investigation on ABA metabolism in GA-induced dormancy in Dioscorea, we isolated from the bulbils of D. japonica the fragments of two homologs ofNCEDs (DjNCED1 & 2) as well as the full sequences of two homologs of ABA8'oxs (DjABA8'ox 1 & 2) which we had already cloned. We preliminarily tried to analyze expression of these ABA metabolic genes in the bulbils of D. japonica at different dormant state. The treatment of uniconazol, which induces the sprouting of half-dormant bulbils, decreased the expression level of DjNCEDl in the bulbils, while increased the level of DjABA8'ox2. This is not contradictory to the hypothesis that GAs keep the endogenous level of ABA higher to induce and maintain bulbil dormancy. However, further detailed analysis of the gene expression levels in the course of dormancy development of the bulbils remains to be needed.
植物化学調節学会の論文
2007-10-05