16 AP2 ドメイン蛋白質をコードするシロイヌナズナ CHOTTO1 遺伝子は種子の ABA 応答と休眠に関与する

概要

論文の詳細を見る
Abscisic acid (ABA) plays key roles in several aspects of seed development, including promotion of seed dormancy and suppression of germination. Arabidopsis chotto1 (cho1) has been identified as an ABA response mutation that preferentially confers resistance to (-)-(R)-ABA, an enantiomer of the natural (+)-(S)-ABA, during seed germination. We show some data that suggest that the control of dormancy and germination is compromised in the mutant. First, mutant seeds can germinate in the presence of 2 μM (+)-ABA that prevent wild-type germination. In the absence of ABA, mutant seeds germinate faster than wild-type seeds. Second, cho1 abi5 digenic mutation increased the resistance to 10 μM (+)-ABA as compared with each monogenic mutation. Third, CHO1 gene whose predicted amino acid sequence contains two AP2 DNA-binding domains expressed predominantly in mature siliques and transiently in germinating seeds of wild type. The effects of the mutation and the gene expression pattern indicate that the CHO1 gene plays a role in regulating seed dormancy and germination, probably in response to endogenous ABA.
植物化学調節学会の論文
2002-10-31