3 ブラシノステロイドの代謝に関わるトマト由来シトクロームP450の機能解析

概要

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Brassinosteroid (BR) am now recognized as a major hormone controlling plant development and growth. Recently several cytochrome P450 genes have been isolated from the mutants deficient in BR (Arabidopsis cpd, dwf4, bas1 and tomato dwarf). The physiological function of these P450s has been proposed from analysis of the corresponding routants in chemical levels. But enzymatic characters of these P450s are not yet clear. Recently, Arabidopsis BAS1 (CYP72B1) has been isolated and suggested to catalyze the C26-hydroxylation of brassinolide. To investigate regulation of BR metabolism in tomato, we have obtained P450 cDNA belonging to CYP72 family from tomato. The tomato cDNA tomato CYP72B, showed 60% identity to BAS 1. in this study, we produced recombinant proteins, which was over-expression of tomato CYP72B in the hetelogous expression system of baculovirus-insect cell. The tomato CYP72B over-expressed protein has typical character of P450 enzyme. Secondary, we cultivated a recombinant tobacco transformed by tomato CYP72B. The transgenic tobacco had typical brassinosteroids deftdent phenotype.
植物化学調節学会の論文
2001-10-09