100. バラ香気成分2-phenylethanolの新規生合成経路の解明(口頭発表,植物化学調節学会第46回大会)

概要

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2-Phenylethanol (2PE) is a prominent scent compound in roses. We have demonstrated that 2PE is produced without loss of the α-position hydrogen from L-[^2H_8]Phenylalanine (L-[^2H_8]Phe) by feeding experiments. In 2008-2011, we found [^2H_7]-2PE production in rose protoplast harvested from May to October. We, here aimed the functional characterization of novel 2PE biosynthetic pathway producing [^2H_7]-2PE. Feeding experiments of L-[^2H_8]Phe to rose protoplast revealed that [^2H_7]-2PE was hardly synthesized in the period from November to April. We characterized two enzymes (aminotransferase and decarboxylase) involving each step in the novel pathway. RNA interference on the biosynthetic enzyme reduced 2PE production. These results suggested that [^2H_7]-2PE is transformed from L-[^2H_8]Phe by the action of aminotransferase and decarboxylase.
2011-10-03