87.植物の化学防御機構におけるイリドイド配糖体糖加水分解酵素の生化学的研究(口頭発表)

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Iridoid glycoside is one of the terpenoid compounds and found in several Oleaceae species such as the olive tree, privet tree. The Olive tree (Olea europaea) is a species of small tree Oleaseae species, and olive fruits is of major agricultural importance as the source of olive oil in the worldwide and pharmacological importance as the protection against cancer and cardiovascular diseases. Olive leaves contain a large amount of the iridoid glycoside, which is stably kept by itself in the vacuoles or cytosol of the leaf cell. Oleuropein is a major iridoid glycoside contained in olive leaf. When the leaf tissue is destroyed by herbivore like olive weevil, oleuropein is activated into a very strong protein denaturant that has glutaraldehyde-like structure by enzyme such as β-D-glucosidase and polyphenoloxidase that localized in organelles separated from the oleuropein. As a result, the activated oleuropein has adverse effects against herbivores by reducing the nutritive value of plant protein. Nevertheless the activation pathway has not been clearly proven yet. To investigate the roll of oleuropein in chemical defense of plant, we isolated a β-D-glucosidase that is a key enzyme from olive fruit and analyzed by useing biochemical techniques. Furthermore, the amino acid sequence of the protein was analyzed by LC-MS/MS. As a result, the purified β-D-glucosidase had high activity against oleuropein and exhibited high-homology with a β-D-glucosidase derives from plant.
2010-10-01

87.植物の化学防御機構におけるイリドイド配糖体糖加水分解酵素の生化学的研究(口頭発表)

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