99. 傷害応答によって生合成されるオレウロペイン糖加水分解産物の構造解析(口頭発表,植物化学調節学会第46回大会)

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Oleuropein, a secoiridoid glycoside, found in several Oleaceae species such as the olive tree and privet tree. Oleuropein has several hydroxyl moieties, caffeoyl group and glucose group. The olive tree (Olea europaea) is a species of small tree in Oleaceae species, and olive fruit is of major agricultural importance in the word as the source of olive oil. Olive leaves and fruits contain up to 8% oleuropein in the wet weight. Oleurpein are stable, does not have any of the activities, and is kept in the vacuoles or cytosol of the leaf cell. With damaged by herbivore, enzymatic activity localized in organelles separate from oleuropein starts to activate oleuropein into a very strong protein denaturant that has glutaraldehyde-like structure by enzyme such as β-glucosidase (β-GH) and polyphenol oxidase that localized in organelles separated from the iridoid glycoside. As a result, the activated iridoid glycoside has adverse effects against herbivores by decreasing the nutritive value of plant protein. Nevertheless the activation pathway has not been clearly proven yet. To investigate the physiological function of iridoid glycoside in chemical defense, we determined chemical structure of oleuropein hydrolyzed metabolite (OHM) by LC-MS/MS and NMR.
2011-10-03