43.バラ主要香気成分生合成に関与するフェニルアセトアルデヒド還元酵素の機能および局在の研究(口頭発表)

概要

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2-Phenylethanol (2PE), main aroma component of rose, is biosynthesized from L-phenylalanine by the action of two enzymes, aromatic L-amino acid decarboxylase and phenylacetaldehyde reductase (PAR). The aim of this study is to elucidate biochemical function of PAR in the 2PE synthesis and latters emission process. First we examined enantio-selectivity of the plant PAR and the E. coli-expressed enzyme. We also analyzed the changes in the epidermal structures of rose petals of Rosa 'Yves Piaget' during the unfurling process, followed by the immunohistochemical analysis of PAR using an antibody against PAR protein. From Western blotting analysis, we confirmed the specificity of the antibody. To investigate the enantio-selectivity of PAR, either pro R or S-[4-^2H] labeled NADPH and phenylacetaldehyde (PAld) were used for the enzymatic reactions. 2PE produced after the reaction was analyzed by GC-MS to determine the ratio of [1-^2H]-2PE/2PE. We also investigated the enantio-selectivity of PAR-catalyzed reaction of ketone. Both E. coli-expressed and plant-derived enzyme enantio-selectively utilized pro-S hydrogen at C-4 of NADPH to yield [1-^2H]-2PE from PAld. We found changes in the epidermal layer by light and electron microscopes during the course of the floral development process of R. 'Yves Piaget'.
植物化学調節学会の論文
2009-10-06