81.生育環境によるバラ主要香気成分2-phenylethanol生合成経路変化の解明(口頭発表)

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2-Phenylethanol (2PE), biosynthesized from L-phenylalanine (L-phe) via phenylacetaldehyde (PAld) by aromatic L-amino acid decarboxylase and phenylacetaldehyde reductase, is a dominant floral scent compound in Damask and hybrid roses. In the present study, we proposed the occurrence of changes in the biosynthetic pathway of 2PE in Rosa 'Yves Piaget' based on the environmental changes. To elucidate the changes in the biosynthetic pathways, we analyzed metabolites related to the biosynthetic pathway of 2PE in rose petals grown in different environment by capillary electrophoresis-time of flight mass spectrometry (CE-TOFMS). The CE-TOFMS analytical data revealed the difference in the amounts of metabolites related to shikimate pathway between the roses harvested in different environment. Moreover, we confirmed the increase in the amount of 2PE after feeding precursors of 2PE to isolated protoplasts from rose petals in different environment conditions. These results suggest that 2PE biosynthesis changes based upon the environment roses were grown. In addition, a new enzyme involved in biosynthesis of 2PE was identified; further purification and characterization of this new enzyme are underway.
2010-10-01