Two O-Methyltransferases Isolated from Flower Petals of Rosa chinensis var. spontanea Involved in Scent Biosynthesis(PLANT BIOTECHNOLOGY)

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概要

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• Rosa chinensis var. spontanea predominantly emits 1,3,5-trimethoxybenzene together with methyleugenol and isomethyleugenol as minor floral scent compounds. Two O-methyltransferases (OMTs), designated as RcOMT1 and RcOMT2, were isolated from rose flower petals using homology-based screening strategies. RcOMT1 efficiently methylated eugenol and isoeugenol to yield volatile methyleugenol and isomethyleugenol, respectively. Furthermore, the mRNA transcripts of RcOMT1 were highly expressed in floral organs and the expression pattern coincided with intracellular content changes of methyleugenol and isomethyleugenol in rose flowers. In contrast, RcOMT2, which shows 94% similarity with caffeic acid O-methyltransferase (COMT) of Prunus amygdalus, was expressed in all tissues tested and had the highest activity with caffeic acid, a typical substrate for COMT. However, this COMT-like OMT also showed some degrees of activity with all three putative precursors of 1,3,5-trimethoxybenzene.

• 社団法人日本生物工学会の論文
• 2003-08-25

著者

• WATANABE Naoharu

  Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

• Watanabe N

  Faculty Of Agriculture Shizuoka University

• Watanabe Naoharu

  Department Of Agricultural Chemistry The University Of Tokyo

• Ueda Yoshihiro

  Laboratory Of Floricultural Science Faculty Of Horticulture Chiba University

• Ueda Yoshihiro

  Laboratory Of Ornamental Plant Science Faculty Of Horticulture Chiba University

• Ebizuka Yutaka

  Department Of Natural Product Chemistry Graduate School Of Pharmaceutical Sciences University Of Tok

• Shibuya Masaaki

  Department Of Natural Product Chemistry Graduate School Of Pharmaceutical Sciences University Of Tok

• WU Shuiqin

  Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

• MITA SATORU

  Institute for Genetic Research and Biotechnology, Shizuoka University

• Mita S

  Institute For Genetic Research And Biotechnology Shizuoka University

• Wu Shuiqin

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

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