Identification of Diterpene Biosynthetic Gene Clusters and Functional Analysis of Labdane-Related Diterpene Cyclases in Phomopsis amygdali
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概要
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Two diterpene biosynthesis gene clusters in the fusicoccin-producing fungus, Phomopsis amygdali, were identified by genome walking from PaGGS1 and PaGGS4 which encode the geranylgeranyl diphosphate (GGDP) synthases. The diterpene cyclase-like genes, PaDC1 and PaDC2, were respectively located proximal to PaGGS1 and PaGGS4. The amino acid sequences of these two enzymes were similar to those of fungal labdane-related diterpene cyclases. Recombinant PaDC1 converted GGDP mainly into phyllocladan-16α-ol via (+)-copalyl diphosphate (CDP) and trace amounts of several labdane-related hydrocarbons which had been identified from the P. amygdali F6 mycelia. Since phyllocladan-16α-ol had not been identified in P. amygdali F6 mycelia, we isolated phyllocladan-16α-ol from the mycelia. Recombinant PaDC2 converted GGDP into (+)-CDP. Furthermore, we isolated the novel diterpenoid, phyllocladan-11α,16α,18-triol, which is a possible metabolite of phyllocladan-16α-ol in the mycelia. We propose that genome walking offers a useful strategy for the discovery of novel natural products in fungi.
- 社団法人 日本農芸化学会の論文
著者
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Toyomasu Tomonobu
Department of Bioresource Engineering, Yamagata University
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MITSUHASHI Wataru
Department of Bioresource Engineering, Yamagata University
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SASSA Takeshi
Department of Bioresource Engineering, Yamagata University
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Shiono Yoshihito
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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TOSHIMA Hiroaki
Department of Bioresource Science, College of Agriculture, Ibaraki University
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OIKAWA Hideaki
Division of Chemistry, Graduate School of Science, Hokkaido University
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KENMOKU Hiromichi
Department of Bioresource Engineering, Yamagata University
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KANNO Yuri
Department of Bioresource Engineering, Yamagata University
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DAIRI Tohru
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University
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KATO Nobuo
The Institute of Scientific and Industrial Research, Osaka University
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NIIDA Rie
Department of Bioresource Engineering, Yamagata University
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MIURA Shigeyoshi
Department of Bioresource Engineering, Yamagata University
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NAKANO Chiaki
Graduate School of Science and Technology, Niigata University
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HOSHINO Tsutomu
Graduate School of Science and Technology, Niigata University
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