Differential Expression of Acid Invertase Genes during Seed Germination in Arabidopsis thaliana
スポンサーリンク
概要
- 論文の詳細を見る
In Arabidopsis thaliana (L.) Heynh ecotype Landsberg, levels of soluble acid invertase activity are closely related to the progress of seed germination. To study the mechanism(s) of the development of these enzymes, two cDNA clones that encode putative vacuolar acid invertases were isolated from germinating seeds and very young seedlings using reverse-transcription polymerase chain reactions with degenerate primers. These fragments corresponded to the genes Atβfruct3 and Atβfruct4 from the Columbia ecotype. An apoplasmic invertase gene corresponding to Atβfruct1/ATCWINV1 was also isolated from these samples. Northern blot analyses showed that Atβfruct3 and Atβfruc4 are expressed concomitantly with germination and the subsequent seedling growth. In contrast, the Atβfruct1/AtcwINV1 mRNA is translated before germination. These expression patterns are regulated by phytochrome, which perceives red light and in turn triggers de novo synthesis of gibberellin, initiating Arabidopsis seed germination. To test the effects of gibberellin on the expression of these genes, seed were treated with a gibberellin biosynthesis inhibitor, uniconazole or prohexadione. These chemicals inhibited both seed germination and expression of the above genes, but subsequently applied GA4, an active gibberellin, reversed the inhibition. These results suggest that the transcription of genes encoding the vacuolar invertases, Atβfruct3 and Atβfruct4 and a gene encoding the apoplasmic invertase, Atβfruct1/AtcwINV1, are induced by gibberellin synthesized de novo following irradiation with red light.
- 社団法人 日本農芸化学会の論文
- 2004-03-23
著者
-
KAMIYA Yuji
Plant Hormone Function, Frontier Research Program, RIKEN
-
Kamiya Y
Plant Science Center Riken
-
Kamiya Y
Riken Plant Science Center
-
Kamiya Yuji
Plant Science Center Riken
-
Toyomasu Tomonobu
Department of Bioresource Engineering, Yamagata University
-
MITSUHASHI Wataru
Department of Bioresource Engineering, Yamagata University
-
Kanazawa Akihiko
Department of Cardiology, New Tokyo Hospital
-
KAMIYA Yuji
RIKEN Plant Science Center
-
Kanazawa Akihiko
Department Of Bioresource Engineering Faculty Of Agriculture Yamagata University
-
Kamiya Y
Riken Kanagawa Jpn
-
Kamiya Yuji
Riken Frp.
-
SASAKI Shigekazu
Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University
-
YANG Young-Yell
Plant Science Center, RIKEN
-
Toyomasu Tomonobu
Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Bi
-
Mitsuhashi Wataru
Forest Biology Division Forestry And Forest Products Research Institute
-
Kamiya Y
Growth Regulation Research Group Riken Plant Science Center
-
Yang Young-yell
Plant Science Center Riken
-
Yang Young-yell
Plant Hormone Function Frp Riken
-
Toyomasu T
Department Of Bioresource Engineering Yamagata University
-
Kamiya Yuji
Plant Hormone Function Frontier Research Program Riken
-
KAMIYA Yuki
Faculty of Agriculture, Niigata University
-
Sasaki Shigekazu
Department Of Bioresource Engineering Faculty Of Agriculture Yamagata University
-
Toyomasu Toshihisa
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
-
MITSUHASHI Wataru
Department of Biology, Tokyo Metropolitan University
関連論文
- TISSUE SPECIFIC EXPRESSION OF GIBBERELLIN UP REGULATED GENES IN AZUKI BEAN EPICOTYLS
- Diterpene Phytoalexins Are Biosynthesized in and Exuded from the Roots of Rice Seedlings
- Emission of ent-Kaurene, a Diterpenoid Hydrocarbon Precursor for Gibberellins, into the Headspace from Plants
- ROBUST VISUAL TRACKING BY INTEGRATING COLOR AND MOTION FEATURES(International Workshop on Advanced Image Technology 2007)
- ROBUST VISUAL TRACKING BY INTEGRATING COLOR AND MOTION FEATURES
- CDNA CLONING OF GIBBERELLIN 20-OXIDASE FROM RICE(Oryza sativa L. )SEEDLINGS
- CLONING OF ent-KAURENE SYNTHASE B cDNA FROM PUMPKIN(Cucurbita maxima L. )
- cDNA CLONING OF ENT-KAURENE SYNTHETASEB FROM THE IMMATURE SEEDS OF PUMPKIN
- Effects of a Plant-Growth Regulator, Prohexadione-Calcium (BX-112), on the Endogenous Levels of Gibberellins in Rice
- Effects of a Plant-Growth Regulator, Prohexadione, on the Biosynthesis of Gibberellins in Cell-Free Systems Derived from Immature Seeds
- Effects of a New Plant Growth Regulator Prohexadione Calcium (BX-112) on Shoot Elongation Caused by Exogenously Applied Gibberellins in Rice (Oryza sativa L.) Seedlings
- Cloning of a Gene Cluster Responsible for the Biosynthesis of Diterpene Aphidicolin, a Specific Inhibitor of DNA Polymerase α
- Gibberellin Is Essentially Required for Carrot (Daucus carota L.) Somatic Embryogenesis : Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions(Biochemistry & Molecular Biology)
- Diterpene Cyclases Responsible for the Biosynthesis of Phytoalexins, Momilactones A, B, and Oryzalexins A-F in Rice
- Comparative Studies on the Arabidopsis Aldehyde Oxidase (AAO) Gene Family Revealed a Major Role of AAO3 in ABA Biosynthesis in Seeds
- Production of Homo-and Hetero-Dimeric Isozymes from Two Aldehyde Oxidase Gense of Arabidopsis thaliana
- INVOLVEMENT OF ARABIDOPSIS ALDEHYDE OXIDASE IN ABA BIOSYNTHESIS
- TWO ALDEHYDE OXIDASE GENES OF ARABIDOPSIS THALIANA ENCODE PEPTIDES FOR THREE DIMERIC ISOZYMES
- Molecular Cloning of a Novel Sex Pheromone Responsible for the Release of a Different Sex Pheromone in Closterium peracerosum-strigosum-littorale Complex
- Molecular Cloning and Characterization of Aldehyde Oxidases in Arabidopsis thaliana
- Analysis of Genomic Sequences Encoding a Sex Pheromone from the Closterium peracerosum-strigosum-littorale Complex
- MOLECULAR ANALYSIS OF ALDEHYDE OXIDASE GENE FROM MAIZE COLEOPTILES
- Deactivation of Gibberellin by 2-Oxidation during Germination of Photoblastic Lettuce Seeds(Organic Chemistry)
- Effects of phytohormones on development of apoplastic specific endopeptidase activities from pea stem
- REGULATION BY LIGHT AND LOCALIZATION OF GENE EXPRESSION OF GIBBERELLIN BIOSYNTHETIC ENZYMES DURING GERMINATION OF PHOTOBLASTIC LETTUCE SEEDS
- Regulation by Phytochrome of Gene Expression of Gibberellin Biosynthetic Enzymes during Germination of Photoblastic Lettuce Seeds
- Effects of the removal of cotyledons on endogenous gibberellin levels in hypocotyls of young cucumber and tomato seedlings
- Contribution of Gibberellin Deactivation by AtGA2ox2 to the Suppression of Germination of Dark-Imbibed Arabidopsis thaliana Seeds
- Differential Expression of Acid Invertase Genes during Seed Germination in Arabidopsis thaliana
- Regulation of Gibberellin Biosynthetic Genes during Arabidopsis Seed Germination
- INVOLVEMENT OF COTYLEDON AND GIBBERELLIN IN THE TISSUE-REUNION PROCESS OF CORTEX IN THE CUT HYPOCOTYL OF CUCUMBER AND TOMATO
- LOCALIZATION OF GENE EXPRESSION OF GIBBERELLIN BIOSTNTHETIC ENZYME IN PHOTOBLASTIC LETTUCE SEEDS
- CLONING AND CHARACTERIZATION cDNAs ENCODING GIBBERELLIN BIOSYNTHETIC ENZYMES FROM LETTUCE
- PHYTOCHROME REGULATION OF GIBBERELLIN 3β-HYDROXYLASE GENES IN GERMINATING ARABIDOPSIS SEEDS
- Ent-KAURENE SYNTHASE B CAN COMPLEMENT ARABIDOPSIS THALIANA ga2 MUTANT
- Gibberellin Biosynthesis in Bambusoideae
- Effect of Ethylene and Gibberellin on the Elongation of Rice Seedlings(Oryza sativa L. )
- Effects of Low Irradiance Stress on Gibberellin Levels in Pea Seedlings : GROWTH AND DEVELOPMENT
- Effects of Gibberellins on Seed Germination of Phytochrome-Deficient Mutants of Arabidopsis thaliana : GROWTH AND DEVELOPMENT
- cDNA CLONING OF ent-KAURENE SYNTHETASEA FROM RICE
- Effect of Ethylene and Gibberellin on theElongation of Rice Seedlings (Oryza sativa L.)
- Effects of Fluorogibberellins on Plant Growth and Gibberellin 3β-Hydroxylases
- Effects of Ethylene and Gibberellins on the Elongation of Rice Seedlings (Oryza sativa L.)
- Inhibition of ent-Kaurene Synthase by Quaternary Ammonium Growth Retardants
- Effects of Gibberellins and Prohexadione on the Activities of Oryzain and α-Amylase in Rice Seeds : PROTEINS, ENZYMES AND METABOLISM
- 12 Activities of natural gibberellins and related compounds in inducing gene expressions of α-amylase and oryzain in rice aleurone tissues
- Effects of 3-Methylgibberellin Analogs on Gibberellin 3β-Hydroxylases and Plant Growth
- Characterization of a Rice Gene Family Encoding Type-A Diterpene Cyclases
- A Step in the Biosynthesis of Gibberellins that Is Controlled by the Mutation in the Semi-Dwarf Rice Cultivar Tan-Ginbozu
- cDNA Cloning and Characterization of Gibberellin-responsive Genes in Photoblastic Lettuce Seeds
- Effects of Exogenously Applied Gibberellin and Red Light on the Endogenous Levels of Abscisic Acid in Photoblastic Lettuce Seeds : GROWTH AND DEVELOPMENT
- Control by Light of Hypocotyl Elongation and Levels of Endogenous Gibberellins in Seedlings of Lactuca sativa L. : GROWTH AND DEVELOPMENT
- Cloning of a Full-length cDNA Encoding ent-Kaurene Synthase from Gibberella fujikuroi : Functional Analysis of a Bifunctional Diterpene Cyclase
- Characterization of an Arabidopsis thaliana Mutant that Has a Defect in ABA Accumulation : ABA-Dependent and ABA-Independent Accumulation of Free Amino Acids during Dehydration
- ANALYSIS OF AN ABSCISIC ACID DEFICIENT MUTANT IN ARABIDOPSIS THALIANA
- A Plant Growth Retardant, Uniconazole, Is a Potent Inhibitor of ABA Catabolism in Arabidopsis
- Ethylene Promotes Submergence-Induced Expression of OsABA8ox1, a Gene that Encodes ABA 8'-Hydroxylase in Rice
- Expression of Two Genes Encoding Gibberellin 2- and 3-oxidases in Developing Seeds of Phaseolus coccineus
- A-12 KAURENE SYNTHASE AND SEED DEPARTMENT
- 57 Prohexadione and Daminozide have similar modes of action as inhibitors of the late stages of gibberellin metabolism.
- BER Performance Improvement of BPSK with Pilot Tone under Frequency-Selective Fading Using Diversity
- Cloning and Characterization of cDNAs Encoding ent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes
- Comparison of the Enzymatic Properties of ent-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice
- Identification of Diterpene Biosynthetic Gene Clusters and Functional Analysis of Labdane-Related Diterpene Cyclases in Phomopsis amygdali
- Sequential Development of Cysteine Proteinase Activities and Gene Expression during Somatic Embryogenesis in Carrot
- Total Synthesis of (±) -syn-Copalol(Organic Chemistry)
- Total Synthesis of (+)-Albicanol and (+)-Albicanyl Acetate
- Genetic Evidence for the Role of Isopentenyl Diphosphate Isomerases in the Mevalonate Pathway and Plant Development in Arabidopsis
- Metabolism of Gibberellins in Cell-Free Extracts of Anthers from Normal and Dwarf Rice
- Bioactive Gibberellin and Its Metabolism in Shoots of Phaseolus vulgaris
- Effects of Deoxygibberellin C (DGO and 16-Deoxo-DGC on Gibberellin 3β-Hydroxylase and Plant Growth
- Conversion of GA_5 to GA_6 and GA_3 in Cell-free Systems from Phaseolus vulgaris and Oryza sativa(Organic Chemistry)
- Partial Purification and Characterization of Gibberellin 3β-hydroxylase from Immature Seeds of Phaseolus vulgaris L
- Metabolism of [^C]GA_ in a Cell-Free System from Developing Seeds of Phaseolus vulgaris L.
- Isolation of a Gibberellin Biosynthesis Inhibitor from Testas of Phaseolus vulgaris L.(Organic Chemistry)
- Gibberellins and Pea Seed Development
- CHOTTO1, a Double AP2 Domain Protein of Arabidopsis thaliana, Regulates Germination and Seedling Growth Under Excess Supply of Glucose and Nitrate
- RECOGNITION OF CIRCULAR ROAD SIGNS BY USING CONIC FITTING AND LOG POLAR MAPPING(International Workshop on Advanced Image Technology 2006)
- Gibberellin A_ Is Converted to Gibberellin A_ in Cultured Cells of Nicotiana tabacum and Catharanthus roseus
- Identification of Diterpene. Biosynthetic Gene Clusters and Functional Analysis of Labdane-Related Diterpene Cyclases in Phomopsis amygdali
- Diterpene Phytoalexins Are Biosynthesized in and Exuded from the Roots of Rice Seedlings
- Comparison of the Enzymatic Properties of ent-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice
- Cloning and Characterization of cDNAs Encoding ent-Copalyl Diphosphate Synthases in Wheat : Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes
- Influence of Axis Removal on Amino-, Carboxy- and Endopeptidase Activities in Cotyledons of Germinating Vigna mungo Seeds
- Effects of a Plant Growth Regulator, Prohexadione Calcium (BX-112), on the Elongation of Rice Shoots Caused by Exogenouslyl Applied Gibberellins and Helminthosporol, Part II
- Studies of Sites of Action of a New Plant Growth Retardant (E)-1-(4-Chlorophenyl )-4,4-dimethyl-2- (1, 2, 4-triazol-1-yl ) -1-penten-3-ol (S-3307) and Comparative Effects of Its Stereoisomers in a Cell-Free System from Cucurbita maxima
- Involvement of the CYP78A Subfamily of Cytochrome P450 Monooxygenases in Protonema Growth and Gametophore Formation in the Moss Physcomitrella patens
- Comprehensive Hormone Profiling in Developing Arabidopsis Seeds : Examination of the Site of ABA Biosynthesis, ABA Transport and Hormone Interactions
- Recent Advances Regarding Diterpene Cyclase Genes in Higher Plants and Fungi
- On active control of impulse noise (信号処理)
- CONTOUR EXTRACTION AND TRACKING OF NONRIGID OBJECTS USING MEAN SHIFT ALGORITHM AND LEVEL SET METHODS(INTERNATIONAL Workshop on Advanced Image Technology 2008)
- SIMULTANEOUS PROJECTIVE RECONSTRUCTION OF VISUAL PRIMITIVES WITH BUNDLE ADJUSTMENT(INTERNATIONAL Workshop on Advanced Image Technology 2008)
- On active control of impulse noise (応用音響)
- RECOGNITION OF CIRCULAR ROAD SIGNS INVARIANT TO THEIR VARIATION IN APPEARANCE(International Workshop on Advanced Image Technology 2007)
- RECOGNITION OF CIRCULAR ROAD SIGNS INVARIANT TO THEIR VARIATION IN APPEARANCE
- ent-KAURENE SYNTHASE A AND SEED DEVELOPMENT
- 12-Oxo-Phytodienoic Acid-Glutathione Conjugate is Transported into the Vacuole in Arabidopsis
- RSOsPR10 Expression in Response to Environmental Stresses is Regulated Antagonistically by Jasmonate/Ethylene and Salicylic Acid Signaling Pathways in Rice Roots
- Thermoinhibition Uncovers a Role for Strigolactones in Arabidopsis Seed Germination
- Tissue-Specific Transcriptome Analysis Reveals Cell Wall Metabolism, Flavonol Biosynthesis and Defense Responses are Activated in the Endosperm of Germinating Arabidopsis thaliana Seeds