Sequential Development of Cysteine Proteinase Activities and Gene Expression during Somatic Embryogenesis in Carrot
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概要
- 論文の詳細を見る
Three bands of proteinase activity (Rf values of 0.5, 0.6, and 0.7) were detected on activity-stained gels after native gel electrophoresis of carrot (Daucus carota L. cv US-Harumakigosun) suspension cells. After the induction of somatic embryogenesis, one activity band (0.7 band) rapidly disappeared; the 0.6 band was absent at the heart-shaped embryo stage. However, the intensity of the 0.5 band increased during embryogenesis. An additional band (0.25 band) appeared after the torpedo-shaped stage. Three bands (0.25, 0.5, and 0.6) were also detected in zygotic seeds. Two activity bands (0.5 and 0.6) were classified as cysteine proteinases based on sensitivities to N-Ethylmaleimide (NEM) or L-3-trans-Carboxyoxirane-2-Carbonyl-L-Leucyl-Agmatine (E-64). To find candidate genes for the cysteine proteinases, we cloned seven cDNAs encoding putative cysteine proteinases from suspension cells and developing somatic embryos. The expression patterns of the seven genes were categorized into three types (Type A, mRNAs increase concomitantly with somatic embryogenesis; Type B, mRNAs decrease quickly in organized cells; Type C, no significant change in transcript level during somatic embryogenesis).
- 社団法人 日本農芸化学会の論文
- 2004-03-23
著者
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Kamiya Y
Riken Plant Science Center
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Toyomasu Tomonobu
Department of Bioresource Engineering, Yamagata University
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MITSUHASHI Wataru
Department of Bioresource Engineering, Yamagata University
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KASHIWAGI Yoshiko
Department of Bioresource Engineering, Yamagata University
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Kamiya Y
Riken Kanagawa Jpn
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Toyomasu Tomonobu
Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Bi
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Mitsuhashi Wataru
Forest Biology Division Forestry And Forest Products Research Institute
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Kamiya Y
Growth Regulation Research Group Riken Plant Science Center
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Kanno Yuri
Department Of Bioresource Engineering Yamagata University
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YAMASHITA Taroh
Department of Bioresource Engineering, Yamagata University
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KONNAI Tomoko
Department of Bioresource Engineering, Yamagata University
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Toyomasu T
Department Of Bioresource Engineering Yamagata University
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KAMIYA Yuki
Faculty of Agriculture, Niigata University
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Toyomasu Toshihisa
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
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Yamashita Taroh
Department Of Bioresource Engineering Yamagata University
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Konnai Tomoko
Department Of Bioresource Engineering Yamagata University
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MITSUHASHI Wataru
Department of Biology, Tokyo Metropolitan University
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