Molecular Cloning of a Novel Sex Pheromone Responsible for the Release of a Different Sex Pheromone in Closterium peracerosum-strigosum-littorale Complex
スポンサーリンク
概要
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A sex pheromone, protoplast-release-inducing protein(PR-IP) inducer, of the Closterium peracerosum-strigosum-littorale complex is known to induce the release of PR-IP, from mating-type plus(mt^+) cells during sexual reproduction. The purtified PR-IP inducer was treated with trypsin to obtain internal peptides for determination of partial amino acid sequences. Using these sequences, oligonucleotides were synthesized and used as primers for the combined reverse transcription-PCR. A 296 bp cDNA fragment was amplified, permitting the cloning of corresponding full length cDNA(CpPI; Closterium peracerosum-strigosum-littorale complex PR-IP inducer). The deduced amino acid sequence of CpPI encodes a protein of 212 amino acid residues of M, 23, 071 whereas portion of the peptide secreted is predicted to have 142 amino acid residues of M, 15, 717 and shows no significant similarity with known proteins. The predicted protein has three possible consensus sequences for asparagine-linked glyosylation site. The CpPI gene was expressed when mating-type minus(mt^-) cells were incubated at a low cell density in the light. Nitrogen deprivation from the medium enhances expression of the CpPI gene. An analysis by genomic Southern hybridization revealed that the cDNA probe hybridized to several DNA fragments obtained from both the genome of mt^- and mt^+ cells. However, in mt^- cells, transcripts for the PR-IP inducer could not be detected by Northern hybridization.
- 日本植物生理学会の論文
著者
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Takio Koji
Riken
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Takeuchi Ken-ichi
Biomolecular Characterization Team Riken
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Takio Koji
Biomolecular Characterization Division Riken
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Kamiya Y
Plant Science Center Riken
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Kamiya Y
Riken Plant Science Center
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Kamiya Yuji
Frp Riken
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Dohmae N
Riken Saitama Jpn
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Dohmae Naoshi
Biomolecular Characterization Team Advanced Technol. Support Div. Riken Advanced Sci. Inst.
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DOHMAE Naoshi
Division of Biomolecular Characterization, The Institute of Physical and Chemical Research (RIKEN)
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Takio Koji
Division Of Biomolecular Characterization The Institute Of Physical And Chemical Research (riken)
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Dohmae N
Riken(inst. Physical And Chemical Res.) Saitama Jpn
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Fukumoto Ryo-hei
Graduate School Of Life Sciences Toyo University
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Dohmae Naoshi
Division Of Biomolecular Characterization Riken
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KAMIYA Yuji
Frontier Research Program, The Institute of Physical and Chemical Research(RIKEN)
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Kamiya Yuji
Riken Frp.
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Kamiya Yuji
Laboratory For Plant-hormone Function Frontier Research Program The Institute Of Physical And Chemic
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Kamiya Yuji
Growth Regulation Research Group Riken Plant Science Center
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SEKIMOTO Hiroyuki
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo
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Fukumoto Ryo-hei
Institute of Biological Sciences, University of Tsukuba
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Fujii Tadashi
Faculty of Sciences, Toyo University
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Fujii Tadashi
Graduate School Of Life Sciences Toyo University
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Fujii Tadashi
Fac. Life Sci. Toyo Univ.
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Kamiya Y
Growth Regulation Research Group Riken Plant Science Center
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Takio K
Biomolecular Characterization Division Riken
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Takio Koji
Division Of Biomolecular Characterization Riken
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Fujii T
Graduate School Of Life Sciences Toyo University
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Sekimoto H
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Kamiya Yuji
Frontier Res.program Riken
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KAMIYA Yuki
Faculty of Agriculture, Niigata University
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Sekimoto Hiroyuki
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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