Melittin-Diacylphosphatidylcholine Interaction Examined by Electrospray Inoization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

スポンサーリンク

概要

• 論文の詳細を見る

• The structure of melittin in the presence of dioleoylphosphatidylcholine (DOPC) was investigated using hydrogen-deuterium (H/D) exchange in conjunction with collision induced dissociation (CID) in an rf-only hexapole ion guide with electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR MS). The deuterium incorporation into backbone amide hydrogens of melittin in the presence of DOPC was analyzed at different time points examining the mass of each fragment ion produced by hexapole-CID. The percentage of deuterium incorporation into the fragments of melittin was less than 45% at initial 10 s of isotope exchange. It increased rapidly as the exchange period was prolonged. After 300 s of incubation in D2O about 85% of amide hydrogens were exchanged with deuterium. When melittin was incubated in D2O in the presence of dodecylphosphocholine (DPC), the rate of isotope exchange was reduced at every time point. In the case of melittin alone, more than 80% amide hydrogens were exchanged with deuterium within 10 s. By comparing these time courses, it seems that the contact with DOPC did not induce melittin to change its conformation. DOPC possibly just shielded the melittin molecule while DPC induced melittin to form some stable conformation, such as helical structure. It was revealed that H/D exchange and MS analysis enabled to study such structural changes of a peptide brought about by the interaction with two types of phospholipid even in the presence of 50-fold amount of lipid.

• 日本質量分析学会の論文
• 2002-04-01

著者

• 明石 知子

  横浜市立大学大学院国際総合科学研究科生体超分子科学専攻

• 明石 知子

  横浜市大 大学院総合理学研究科

• Takeuchi Ken-ichi

  Biomolecular Characterization Team Riken

• Takio Koji

  Graduate School Of Integrated Science Yokohama City University

• AKASHI Satoko

  Division of Biomolecular Characterization, The Institute of Physical and Chemical Research (RIKEN)

• Akashi Satoko

  Division Of Biomolecular Characterization The Institute Of Physical And Chemical Research (riken)

関連論文

• レーザースプレー質量分析が可能にした生体高分子複合体の結合親和性の定量的な解析
• Expression of a Novel 90-kDa Protein, Lsd90, Involved in the Metabolism of Very Long-chain Fatty Acid-containing Phospholipids in a Mitosis-defective Fission Yeast Mutant
• 2つのソフトイオン化法の開発 : 2002年度ノーベル化学賞 田中耕一氏、John B.Fenn 教授の業績
• 重水素交換とMSを組み合わせたタンパク質の高次構造解析
• ASMSリポート : タンパク質の構造解析
• 質量分析を用いたタンパク質の構造解析
• ASMSリポート その2
• 巨大タンパク質複合体の質量分析
• 難結晶性生体超分子複合体の溶液構造を解析する : 様々な分析手法を駆使して基本転写因子TFIIEの構造を解析
• 生体高分子の質量分析による構造機能研究
• タンパク質の構造解析法としての質量分析
• 田中耕一氏の発見が生命科学研究をどう変えたか
• 田中耕一氏 2002年ノーベル化学賞受賞
• 質量分析によるタンパク質-タンパク質およびタンパク質-RNA複合体の構造研究 : X線結晶構造解析と質量分析で見えてくる生体超分子の構造
• 1P-056 超遠心分析沈降速度法を用いたTRAP-antiTRAP相互作用解析(蛋白質・構造機能相関(1),第46回日本生物物理学会年会)
• Conformational Changes of Proteins Observed by Hydrogen/Deuterium Exchange and Electrospray Ionization Mass Spectrometry
• Melittin-Diacylphosphatidylcholine Interaction Examined by Electrospray Inoization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
• Evaluation of Binding Affinity of N-Terminally Truncated Forms of Cystatin for Papain with Electrospray Ionization Mass Spectrometry
• Production of Homo-and Hetero-Dimeric Isozymes from Two Aldehyde Oxidase Gense of Arabidopsis thaliana
• TWO ALDEHYDE OXIDASE GENES OF ARABIDOPSIS THALIANA ENCODE PEPTIDES FOR THREE DIMERIC ISOZYMES
• Molecular Cloning of a Novel Sex Pheromone Responsible for the Release of a Different Sex Pheromone in Closterium peracerosum-strigosum-littorale Complex
• MOLECULAR ANALYSIS OF ALDEHYDE OXIDASE GENE FROM MAIZE COLEOPTILES
• A Monoclonal Antibody, 3A10, Recognizes a Specific Amino Acid Sequence Present on a Series of Developmentally Expressed Brain Proteins
• Localization of the Phosphatidylserine-Binding Site of Glyceraldehyde-3-Phosphate Dehydrogenase Responsible for Membrane Fusion
• P-405 SITE-SPECIFIC FUNCTIONALIZATION OF PROTEINS BY ORGANOPALLADIUM REACTIONS
• Detection of Molecular Ions of Non-Covalent Complexes of Ras・GDP and Ras・GppNp by MALDI-TOFMS
• Characterization of a Thermostable Lysine-Specific Metalloendopeptidase from the Fruiting Bodies of a Basidiomycete, Grifola frondosa
• Kinetic Characterization of Lysine-Specific Metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus Fruiting Bodies
• Site-specific incorporation of 4-lodo-L-phenylalanine through opal suppression

もっと見る 閉じる

スポンサーリンク