Effects of the removal of cotyledons on endogenous gibberellin levels in hypocotyls of young cucumber and tomato seedlings

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概要

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• We have previously shown that gibberellin (GA) is required for tissue-reunion in the cortex of cut hypocotyls in cucumber (Cucumis sativus) and tomato (Solanum lycopersicum) seedlings, and that intact cotyledons are also necessary for this process. These results suggested that cotyledons might play an important role in controlling GA levels in the hypocotyl in these plant species. In this study, we found that a local application of a GA biosynthesis inhibitor uniconazole to cotyledons was effective to inhibit hypocotyl elongation, and that simultaneous application of GA canceled this inhibition. To study the role of cotyledons in GA content in the hypocotyl directly, cotyledons were removed from 7-days-old seedlings and endogenous GA levels in the hypocotyl were determined. Our results demonstrated that the cotyledon-removed seedlings contained lower levels of bioactive GAs and their precursors in the hypocotyls than did intact seedlings. Quantitative RT-PCR analysis indicated that transcript levels of LeGA20ox1 and LeGA3ox2 genes were elevated in the hypocotyl after the removal of cotyledons, suggesting that the reduced bioactive GA levels caused upregulation of these genes via the feedback regulation mechanism. Taken together, our results suggest that cotyledons are necessary for maintaining normal GA levels in young cucumber and tomato hypocotyls.

• 日本植物細胞分子生物学会の論文
• 2007-03-01

著者

• Kamiya Y

  Riken Plant Science Center

• Kamiya Yuji

  Plant Science Center Riken

• Higashi K

  Gene Research Center Institute Of Biological Sciences University Of Tsukuba

• KAMADA Hiroshi

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• KAMIYA Yuji

  RIKEN Plant Science Center

• YAMAGUCHI Shinjiro

  RIKEN Plant Science Center

• Kamiya Y

  Riken Kanagawa Jpn

• Kamiya Yuji

  Riken

• Kamada H

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• ASAHINA Masashi

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• YAMAUCHI Yukika

  RIKEN Plant Science Center

• HANADA Atsushi

  RIKEN Plant Science Center

• SATOH Shinobu

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• 朝比奈 雅志

  帝京大・バイオサイエンス:筑波大・院・生命環境:理研・psc

• 朝比奈 雅志

  帝京大・バイオ

• Kamada Hiroshi

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Yamaguchi Shinjiro

  Plant Science Center Riken

• Yamaguchi Shinjiro

  Frontier Research Program Riken

• Satoh Shinobu

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Satoh Shinobu

  Graduate School Of Life And Environmental Science University Of Tsukuba

• Kanno Yuri

  Department Of Bioresource Engineering Yamagata University

• Hanada Atsushi

  Riken Yokohama Jpn

• Yamaguchi S

  Riken Plant Science Center

• Kawaide Hiroshi

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology

• KAMIYA Yuki

  Faculty of Agriculture, Niigata University

• Kamada Hiroshi

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Kamada Hiroshi

  Graduate School Of Life And Environmental Sciences Gene Research Center University Of Tsukuba

• 朝比奈 雅志

  帝京大学・理工学部・バイオサイエンス学科

• Satoh Shinobu

  Graduate School Of Life And Environmental Sci. Univ. Of Tsukuba

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