Molecular Cloning and Characterization of Aldehyde Oxidases in Arabidopsis thaliana

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概要

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• Using degenerate primers designed by deduced amino acid sequences of known aldehyde oxidases (AO) from maize and bovine, two independent cDNA fragments were amplified by reverse transcription-polymerase chain reaction (PCR). The two corresponding full-length cDNAs (atAO-1 and atAO-2; 4, 484 and 4, 228 bp long, respectively) were cloned by screening the Arabidopsis cDNA library followed by rapid amplification of cDNA end-PCR. These cDNAs are highly homologous at both the nucleotide and amino acid sequence levels, and the deduced amino acid sequences showed high similarity with those of maize and tomato AOs. They contain consensus sequences for two iron-sulfur centers and a molybdenum cofactor (MoCo)-binding domain. In addition, another cDNA having a sequence similar to that of the cDNAs was screened (atAO-3; 3, 049 bp), and a putative AO gene (AC002376) was reported on chromosome 1, which (atAO-4) was distinct from, but very similar to, the above three AOs. atAO-1, 2, 3, and 4 were physically mapped on chromosomes 5, 3, 2 and 1, respectively. These data indicate that there is an AO multigene family in Arabidopsis. atAO-1 protein was shown to be highly similar to one of the maize AOs in respect to a region thought to be involved in determination of substrate specificity, suggesting that they might encode a similar type of AO, which could efficiently oxidize indole-3-acetaldehyde to indole-3-acetic acid (IAA). atAO-1 and atAO-2 genes were expressed at higher levels in lower hypocotyls and roots of the wild-type seedlings, while atAO-3 was slightly higher in cotyledons and upper hypocotyls. The expression of atAO-1 was more abundant in the seedlings of an IAA overproducing mutant (superroot1;sur1) than in those wild type. atAO-2 and atAO-3 transcripts were rather evenly distributed in these seedlings. A possible involvement of atAO genes in phytohormone biosynthesis in Arabidopsis is discussed.

• 日本植物生理学会の論文

著者

• Kamiya Y

  Plant Science Center Riken

• Kamiya Y

  Riken Plant Science Center

• Kamiya Yuji

  Frp Riken

• SEKIMOTO Hiroyuki

  Frontier Research Program, The Institute of Physical and Chemical Research(RIKEN)

• KAMIYA Yuji

  Frontier Research Program, The Institute of Physical and Chemical Research(RIKEN)

• Kamiya Yuji

  Riken Frp.

• Kamiya Yuji

  Laboratory For Plant-hormone Function Frontier Research Program The Institute Of Physical And Chemic

• Kamiya Yuji

  Growth Regulation Research Group Riken Plant Science Center

• KOSHIBA Tomokazu

  Department of Biological Sciences, Tokyo Metropolitan University

• SEO Mitsunori

  Department of Biology, Tokyo Metropolitan University

• KOMANO Teruya

  Department of Biology, Tokyo Metropolitan University

• Kawakami Naoto

  Faculty of Agriculture, Meiji University

• Desloire Sophie

  Laboratoire de Biologie Cellulaire et Moleculaire, Institut National de la Recherche Agronomique

• Liotenberg Sylviane

  Laboratoire de Biologie Cellulaire et Moleculaire, Institut Nastional de la Recherche Agronomique

• Marion-Poll Annie

  Laboratoire de Biologie Cellulaire et Moleculaire, Institut Nastional de la Recherche Agronomique

• Caboche Michel

  Laboratoire de Biologie Cellulaire et Moleculaire, Institut Nastional de la Recherche Agronomique

• SEO Mitsunori

  RIKEN Plant Science Center

• Caboche Michel

  Laboratoire De Biologie Cellulaire

• Caboche Michel

  Laboratoire De Biologie Cellulaire Et Moleculaire Institut Nastional De La Recherche Agronomique

• Kamiya Y

  Growth Regulation Research Group Riken Plant Science Center

• Komano Teruya

  Department Of Biological Sciences Tokyo Metropolitan University

• Seo Mitsunori

  Department Of Biological Sciences Tokyo Metropolitan University

• Kawakami Naoto

  Faculty Of Agriculture Meiji University

• Sekimoto H

  Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University

• Koshiba T

  Growth Regulation Research Group Riken Plant Science Center

• Koshiba Tomokazu

  Department Of Bio!ogy Tokyo Metropolitan University

• Kamiya Yuji

  Frontier Res.program Riken

• KAMIYA Yuki

  Faculty of Agriculture, Niigata University

• Desloire Sophie

  Laboratoire De Biologie Cellulaire Et Moleculaire Institut National De La Recherche Agronomique

• Sekimoto Hiroyuki

  Frontier Research Program The Institute Of Physical And Chemical Research(riken)

• Marion-poll Annie

  Laboratoire De Biologie Cellulaire Et Moleculaire Institut Nastional De La Recherche Agronomique

• Liotenberg Sylviane

  Laboratoire De Biologie Cellulaire Et Moleculaire Institut Nastional De La Recherche Agronomique

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