Effects of Temperature on Growth and Protein and RNA Syntheses of Excised Vigna mungo Embryonic Axis

概要

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Embryonic axes excised from dry Vigna mungo seeds cultivated on wet filter paper grew well at 27℃, but did poorly below 15℃. The increase in fresh weight at 27℃ was completely inhibited by both cycloheximide and α-amanitin. Excised axes could grow well at 15℃ only if cultivated at 27℃ for the first 12h. Also, excised axes cultivated at 15℃ for several days grew well when transferred to 27℃. These results suggest that the ability of the axes to grow is retained during cultivation at 15℃, even when no growth occurs, and that only the initial stage of cultivation requires high temperatures for growth. The incorporation of ^3H-leucine into protein in the excised axes during the first 6 h of cultivation at 15℃ was much slower than that at 27℃. The incorporation rate at 15℃ during the second 6 h was, however, considerably high. In contrast, the incorporation of ^3H-uridine into the total RNA fraction at 15℃ was much slower during the first and the second 6 h as compared to that at 27℃, suggesting a difference in the response to temperature between protein and RNA syntheses in the axes.
日本植物生理学会の論文