Identification of Regulatory Elements Involved in Expression of a Rice Endopeptidase Gene Rep1 in Embryos
スポンサーリンク
概要
- 論文の詳細を見る
A gene for a cysteine endopeptidase REP-1, Rep1, is expressed in both the embryo and aleurone layers in germinating rice seeds. The expression of Rep1 was not repressed in the embryo by exogenously applied uniconazole, a gibberellin (GA) biosynthesis inhibitor, even though GA-inducible expression of the gene occurred in the aleurone layers. Serially truncated Rep1 promoters fused to the β-glucuronidase (GUS) reporter gene were employed to identify the cis-regulatory elements involved in the embryo-specific expression. The deletion of the region from -500 to -432 decreased the GUS activity significantly. Linker-scan mutation analysis was carried out to determine the sequences involved in the expression of Rep1 in embryos, which showed that the region from -500 to -432 contained at least two positive regulatory elements.
- 日本植物細胞分子生物学会の論文
- 2003-06-01
著者
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Yamauchi Daisuke
Department Of Life Science Graduate School Of Life Science University Of Hyogo
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SUTOH Keita
Department of Biological Sciences, Tokyo Metropolitan University
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Sutoh Keita
Department Of Biological Sciences Tokyo Metropolitan University
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Sutoh Keita
Dept. Biol. Sci. Tokyo Metropolitan Univ.
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MINAMIKAWA Takao
Dept. Biol. Sci., Tokyo Metropolitan Univ.
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MINAMIKAWA Takao
Department of Biology, Tokyo Metropolitan University
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Yamauchi Daisuke
Department Of Biological Sciences Tokyo Metropolitan University
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Minamikawa Takao
Department Of Biological Sciences Tokyo Metropolitan University
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Kato Hideki
Winter Stress Laboratory National Agricultual Research Center For Hokkaido Region (narch). Naro
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Yamauchi D
Himeji Inst. Of Technol. Hyogo Jpn
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Minamikawa Takao
Departmen T Of Biology Tokyo Metropolitan University
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Yamauchi Daisuke
Department Of Biological Science Graduate School Of Science Tokyo Metropolitan University
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Sutoh Keita
Dept. Biol. Sci., Tokyo Metropolitan Univ.
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