Synthesis and Turnover of α-Amylase in Cotyledons of Germinating Vigna mungo Seeds : Effects of Exogenously Applied End-Products and plant Hormones : PROTEINS, ENZYMES AND METABOLISM

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Pulse-chase experiments indicated that the higher levels of α-amylase in detached and incubated cotyledons of Vigna mungo than those in cotyledons attached to the embryonic axis were due to both faster synthesis and slower degradation of the enzyme in the detached cotyledons than in the attached cotyledons. Levels of α-amylase in the cotyledons were examined in terms of possible effects of end-products and the effects of exogenously applied plant hormones and growth regulators. Levels of α-amylase activity and content were reduced by high concentrations of glucose and sucrose, and it is suggested that this effect was caused mostly by osmotic stress and partly by end-product repression. The level of α-amylase was nearly twice that in controls after 1 to 10 μM GA_3 had been applied to the cotyledons. In addition, 0.1 mM kinetin, 0.1 mM 2,4-D and 0.1 to 0.5 mM naphthaleneacetic acid also increased the level by 34% to 66% as compared to the control. ABA and uniconazole both prevented the synthesis of α-amylase.
日本植物生理学会の論文