In vivo Studies on the Occurrence of Stored mRNA in Embryonic Axes of Vigna unguiculata Seeds

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α-Amanitin and cordycepin at various concentrations were tested for their inhibitory effect on the fresh weight increase of Vigna unguiculata embryonic axes after the onset of imbibition and on the incorporation rate of ^3H-labeled leucine into protein in axes of the 36-38 h stage. α-Amanitin at 0.5-5 μg/ml clearly exerted an inhibitory effect on both the fresh weight increase and the protein synthesis. This drug at 1 μg/ml, however, showed no significant effect on the protein synthesis at an early stage of imbibition (4 h), whereas cycloheximide was a very potent inhibitor. By experiments in which 'dry' axes were allowed to imbibe ^3H-lebeled adenosine solution for 4 and 12 h in the presence of α-amanitin, it was found that poly A^+RNA was newly synthesized to some extent in axes as early as 4 h after the onset of imbibition and that the drug effectively inhibited the poly A^+RNA synthesis. The results may indicate the occurrence of stored mRNA in embryonic axes of V. unguiculata seeds.
日本植物生理学会の論文