Formation of multiple forms of acid and alkaline phosphatases in relation to the culture age of Aspergillus niger
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概要
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Acid phosphatase (EC 3.1.3.2) was extracted from mycelia of Aspergillus niger, then separated and purified into four fractions. These acid phosphatases, designated IA, IB, II and III, had pH optima at 5.0, 4.5-5.0, 4.5 and 2.5, respectively. None required the presence of divalent cations, and all were strongly inhibited by NaF. They were non-specific acid phosphatases but varied in their activities with various substrates. The alkaline phosphatase (EC 3.1.3.1) of A. niger was also separated into two fractions, alkaline phosphatases I and II. Changes in the activity ratios of these acid and alkaline phosphatases were studied during culture in a peptone medium. The activity of acid phosphatase II was higher than the others when the culture was young. The activity of acid phosphatase III increased to a maximum in the actively growing phase, then decreased. That of acid phosphatase I became highest in the mature culture. In contrast, the activity of alkaline phosphatase I was higher than the others in young cultures, while alkaline phosphatase II became dominant in the mature culture. Activities of the various acid and alkaline phosphatases in different regions of the growing colonies were also studied. The changing patterns of these enzymes in both liquid and surface cultures were compared. When A. niger was cultured in a medium containing a low concentration of phosphate, acid phosphatase activity greatly increased after the consumption of phosphate, but alkaline phosphatase activity did not.
- 日本植物生理学会の論文
著者
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Komano Teruya
Department Of Biological Sciences Tokyo Metropolitan University
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Komano Teruya
Department Of Biology Faculty Of Science Tokyo Metropolitan University
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