Comparison and Characterization of cis-Regulatory Regions in Some Embryo-Specific and ABA-Responsive Carrot Genes, DcECPs
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概要
- 論文の詳細を見る
Expression of carrot ECP (DcECP) genes encoding late-embryogenesis abundant proteins is embryo-specific and ABA-inducible. The expression is regulated by C-ABI3, a carrot homologue of Arabidopsis ABI3. To understand the molecular mechanisms controlling ABA-inducible and embryo-specific gene expression, we compared and analyzed the promoter regions of some ECP genes. The accumulation of DcECPs mRNA in response to ABA was not inhibited by an inhibitor of protein synthesis, cycloheximide. These results indicate that the ABA-inducible expression of the DcECPs did not require de novo protein synthesis. Sequence comparison among some ECP gene promoters revealed that the promoters contained several conserved motifs including ABRE (ABA responsive element)-like ACGT core motifs, and an Sph box (CATGCATG), which has been identified as a motif mediating gene activation of maize antocyanin regulatory gene C1. To investigate the promoter activity of DcECP31 promoter region, we carried out a deletion analysis with a transient assay system using protoplasts of embryogenic cells or with a transformation system using embryogenic cells of carrot. We found that the -250bp upstream region of the DcECP31 promoter is sufficient for embryo-specific and ABA-inducible promoter activity. Following deletion analysis of DcECP40 promoter, we clarified that the distal (-670∼-390) and proximal regions (-140∼-50), are essential for the ABA-inducible expression.
- 日本植物細胞分子生物学会の論文
- 2001-03-01
著者
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Ko Sukmin
Gene Research Center Institute Of Biological Sciences University Of Tsukuba
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Kamada H
Univ. Tsukuba Tsukuba Jpn
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KAMADA Hiroshi
Gene Research Center, Graduate School of Life and Environmental Science, University of Tsukuba
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EUN Chang-Ho
Gene Research Center, Institute of Biological Sciences, University of Tsukuba
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HIGASHI Katsumi
Gene Research Center, Institute of Biological Sciences, University of Tsukuba
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Eun Chang-ho
Gene Experiment Center Institute Of Biological Sciences University Of Tsukuba
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Eun Changho
Gene Research Center Institute Of Biological Sciences University Of Tsukuba
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Higashi K
Gene Research Center University Of Tsukuba
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Higashi K
Gene Research Center Institute Of Biological Sciences University Of Tsukuba
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Higashi Katsumi
Gene Experiment Center Institute Of Biological Sciences University Of Tsukuba
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THITAMADEE Siripong
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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YANG Heping
Laboratory Bioactive Natural Product Chemistry, Graduate School of Bio-Agricultural Sciences, Nagoya
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SAGE ONO
Gene Experiment Center, Institute of Biological Sciences, University of Tsukuba
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SATOH Shinobu
Gene Experiment Center, Institute of Biological Sciences, University of Tsukuba
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Sage Ono
Gene Experiment Center Institute Of Biological Sciences University Of Tsukuba
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Yang Heping
Laboratory Bioactive Natural Product Chemistry Graduate School Of Bio-agricultural Sciences Nagoya U
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Kamada H
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Satoh Shinobu
Gene Experiment Center Institute Of Biological Sciences University Of Tsukuba
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Kamada Hiroshi
Gene Experiment Center University Of Tsukuba
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Thitamadee Siripong
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Satoh Shinobu
Univ. Tsukuba Ibaraki Jpn
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Kamada Hiroshi
Gene Exp. Center Univ. Tsukuba
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