Cysteine Proteinase Gene Expression in the Endosperm of Germinating Carrot Seeds(Biochemistry & Molecular Biology)

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概要

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• A gene for carrot seed cysteine proteinase (CSCP ; AB057371, AB057372) was cloned using PCR. The deduced amino acid sequence of CSCP had active sites for eukaryotic cysteine proteinases, putative signal sequences, and an endoplasmic reticulum targeting sequence. RNA gel blot analysis showed that CSCP transcripts appeared from dry seed, reached a maximum 3 days after imbibition (DAI), and disappeared by 5 DAI. In situ hybridization showed that CSCP transcripts accumulated in the endosperm of germinating seeds. This is the first report of the expression pattern of a cysteine proteinase gene in the endosperm of germinating dicotyledonous plants. The promoter of the CSCP gene had an endosperm motif and many other motifs also found in the promoters of endosperm-specific storage protein genes in monocotyledons. It is suggested that dicotyledons, like monocotyledons, have a temporal and spatial regulation system for endosperm-specific gene expression in germinating seeds.

• 社団法人日本農芸化学会の論文
• 2001-10-23

著者

• Kamada H

  Univ. Tsukuba Tsukuba Jpn

• KAMADA Hiroshi

  Institute of Biological Sciences, University of Tsukuba

• SATOH Shinobu

  Institute of Biological Sciences, University of Tsukuba

• YAMAKAWA Seiyei

  Institute of Biological Sciences, University of Tsukuba

• SHEN Shihua

  Institute of Biological Sciences, University of Tsukuba

• Higashi K

  Gene Research Center University Of Tsukuba

• Higashi K

  Gene Research Center Institute Of Biological Sciences University Of Tsukuba

• Satoh S

  Tohoku Univ. Sendai Jpn

• Yamakawa Seiyei

  Graduate School Of Biological Science Nara Institute Of Science And Technology

• Yamakawa Seiyei

  Institute Of Biological Sciences University Of Tsukuba

• Yamakawa Seiyei

  Technology Department Research Association For Biotechnology

• Sakagami Y

  Nagoya Univ.

• Kamada H

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Satoh S

  Institute Of Biological Sciences University Of Tsukuba

• Satoh Shinobu

  Institute Of Biological Sciences University Of Tsukuba

• Shen Shihua

  Gene Research Center University Of Tsukuba

• Oda Atsushi

  Institute Of Biological Sciences University Of Tsukuba

• Kamada Hiroshi

  Institute Of Biological Sciences Univ.

• Sakuta Chiyoko

  Institute of Biological Sciences, University of Tsukuba

• KONISHI Minoru

  Institute of Biological Sciences, University of Tsukuba

• Konishi Minoru

  Institute Of Biological Sciences University Of Tsukuba

• Sakuta C

  Institute Of Biological Sciences University Of Tsukuba

• Sakuta Chiyoko

  Institute Of Biological Sciences University Of Tsukuba

• Kawaide Hiroshi

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology

• Oda A

  Institute Of Biological Sciences University Of Tsukuba

• Hoshino Kaori

  Showa Women's University

• Sakagami Youji

  Laboratory Of Bioactive Natural Product Chemistry Graduate School Of Bio-agricultural Sciences Nagoy

• Yamakawa Seiyei

  School Of Agricultural Sciences Nagoya University

• Satoh Shinobu

  Institute Of Biological Sciences The University Of Tsukuba

• Kamada Hiroshi

  Institute Of Biological Sciences Tsukuba University

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