Isolation of Arabidopsis mutants that have altered sensitivity to staurosporine and ABA
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概要
- 論文の詳細を見る
We have developed a sensitive system to investigate the possible roles of protein phosphorylation in ABA signaling in Arabidopsis. We used staurosporine, a protein kinase inhibitor, to suppress the inhibitory effects of ABA on seed germination and the opening/greening of cotyledons. Using staurosporine, we performed a large genetic screen for asa (altered sensitivity to staurosporine and ABA) mutants and identified novel weak alleles of abi4 and abi5 mutants as asa1 and asa2 mutants. This method will be useful in isolating weak alleles of mutants and in selecting mutants with altered sensitivity to chemical compounds.
- 日本植物細胞分子生物学会の論文
著者
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KAMADA Hiroshi
Institute of Biological Sciences, University of Tsukuba
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SHINOZAKI Kazuo
Institute of Biological Sciences, University of Tsukuba
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Mizoguchi Tsuyoshi
Institute Of Biological Sciences University Of Tsukuba
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Kikuchi Akira
Institute of Biological Sciences, Tsukuba University
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Kato Takaharu
Institute of Biological Sciences, Tsukuba University
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- Isolation of Arabidopsis mutants that have altered sensitivity to staurosporine and ABA