Xylem Sap Lectin, XSP30, Recognizes GlcNAc Sugar Chains of Glycoproteins in Cucumber Leaves

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概要

• 論文の詳細を見る

• The lectin activity of 30-kDa xylem sap protein (XSP30) found in cucumber (Cucumis sativas L.) xylem sap was analyzed by lectin blot coupled with immunological detection of XSP30. XSP30 is homologous to galactose-binding lectin, but it had only weak binding activity against asialofetuin, an animal glycoprotein with a terminal galactose. This reaction was not reduced by galactosidase treatement of asialofetuin. XSP30 bound strongly to soybean agglutinin, whose sugar chains consist solely of mannose and N-acetylglucosamine (GlcNAc), but bound only weakly to soybean peroxidase and γ-globulin, whose GlcNAc is fucosylated. The binding activity was inhibited by tri-N-acetylchitotriose (GlcNAc)3. Leaf parenchyma cells were stained with XSP30 and aniti-XSP30 antibody; this staining was reduced by proteinase treatment of the sections, and XSP30 bound to proteins in leaf particulate fraction. These results suggest that XSP30 transported from root via xylem sap binds to chitobiose, GlcNAc-GlcNAc, in N-linked glycans of leaf glycoproteins.

• 日本植物細胞分子生物学会の論文
• 2003-03-03

著者

• Kamada H

  Univ. Tsukuba Tsukuba Jpn

• KAMADA Hiroshi

  Institute of Biological Sciences, University of Tsukuba

• SATOH Shinobu

  Institute of Biological Sciences, University of Tsukuba

• SHEN Shihua

  Institute of Biological Sciences, University of Tsukuba

• Higashi K

  Gene Research Center University Of Tsukuba

• Higashi K

  Gene Research Center Institute Of Biological Sciences University Of Tsukuba

• Satoh S

  Tohoku Univ. Sendai Jpn

• Kamada H

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Satoh S

  Institute Of Biological Sciences University Of Tsukuba

• Satoh Shinobu

  Institute Of Biological Sciences University Of Tsukuba

• Shen Shihua

  Gene Research Center University Of Tsukuba

• Oda Atsushi

  Institute Of Biological Sciences University Of Tsukuba

• Kamada Hiroshi

  Institute Of Biological Sciences Univ.

• Sakuta Chiyoko

  Institute of Biological Sciences, University of Tsukuba

• Sakuta C

  Institute Of Biological Sciences University Of Tsukuba

• Sakuta Chiyoko

  Institute Of Biological Sciences University Of Tsukuba

• Kawaide Hiroshi

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology

• Oda A

  Institute Of Biological Sciences University Of Tsukuba

• Hoshino Kaori

  Showa Women's University

• Satoh Shinobu

  Institute Of Biological Sciences The University Of Tsukuba

• Kamada Hiroshi

  Institute Of Biological Sciences Tsukuba University

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