Improvement of the Specificity of an Antiserum Raised against a Carrot Glycoprotein by Eliminating the Anti-glycan Antibody(Biological Chemistry)
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概要
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The high immunogenecity of xylose-containing glycans of plant glycoproteins caused a non-specific antiserum which reacted to numerous glycoproteins of carrot cells and bromelain on immunoblots. The antiserum raised against a carrot M_r-57000 glycoprotein was fractionated by affinity chromatography, using a column in which bromelain was coupled with CNBr-Sepharose 4B. The pass-through and bound fractions contained anti-polypeptide moiety and anti-glycan moiety antibodies, respectively. The use of the pass-through fraction greatly improved the specificity when immunoblotting the total carrot proteins.
- 社団法人日本農芸化学会の論文
著者
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SATOH Shinobu
Institute of Biological Sciences, University of Tsukuba
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Satoh Shinobu
Institute Of Biological Sciences The University Of Tsukuba
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