Presence of Organelle-Associated Serine Enzymes in Spinach Leaves
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概要
- 論文の詳細を見る
Particulate fractions prepared from spinach leaves by differential centrifugation were analyzed for proteins capable of binding di-isopropyl phosphorofluoridate (DFP) ; [3^H]-labeled DFP and SDS-polyacrylamide gel electrophoresis were used. The chloroplast-rich fraction contained one kind of DFP-binding protein, whose binding was inhibited by phenylmethylsulfonyl fluoride (PMSF). The mitochondrion-rich fraction contained another DFP-binding protein, whose binding was sensitive to PMSF and L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK). The microsome-rich fraction contained three PMSF-sensitive DFP-binding proteins ; one was sensitive to both N-p-tosyl-L-lysine chloromethyl ketone (TLCK) and TPCK, one was sensitive to TLCK and one to TPCK. These DFP-binding proteins are believed to be serine proteases.
- 日本植物生理学会の論文
著者
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Fujii Tadashi
Institute Of Biological Sciences University Of Tsukuba
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Satoh Shinobu
Institute Of Biological Sciences The University Of Tsukuba
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Fujii Tadashi
Institute Of Biological Science The University Of Tsukuba
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