Intracellular hydroxyproline-rich glycoprotein of suspension-cultured tobacco cells
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概要
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Two soluble glycoproteins containing hydroxyproline were extracted from cultured tobacco cells (cell line XD-6S) and purified by ion-exchange and gel-filtration chromatography. On DEAE-cellulose column chromatography in the final step of the purification, one was eluted at 90 mM NaCl and the other at 120 mM as single peak. Both purified glycoproteins were also sedimented as single peak with an ultracentrifugation. The S_<20,W> values were 6.1 for the former and 7.0 for the latter. These glycoproteins were composed of 94% polysaccharide and 6% protein in the former, and 87% polysaccharide and 13% protein in the latter. The sugar moiety consisted of galactose, arabinose, rhamnose, and uronic acid in both. Hydroxyproline accounted for 12% in the former and 20% in the latter amino acid composition. A high content of alanine in both (14 and 15%) was one of the distinctive characteristics of these soluble glycoproteins. These intracellular soluble hydroxyproline-containing glycoproteins were not label-led within 30 min of incubation with ^3H-proline, although the radioactivity was rapidly incorporated (within 15 min) into the intracellular macromolecules.
- 日本植物生理学会の論文
著者
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Hori Hidetaka
Institute Of Biological Science The University Of Tsukuba:(present)department Of Biochemistry Univer
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Hori Hidetaka
Institute Of Biological Sciences The University Of Tsukuba
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Fujii Tadashi
Institute Of Biological Sciences University Of Tsukuba
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Fujii Tadashi
Institute Of Biological Sciences The University Of Tsukuba
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Fujii Tadashi
Institute Of Biological Science The University Of Tsukuba
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