Purification and Characterization of a Novel Sex Pheromone that Induces the Release of Another Sex Pheromone during Sexual Reproduction of the Heterothallic Closterium peracerosum-strigosum-littorale Complex : PROTEINS, ENZYMES AND METABOLISM : CELL-CELL
スポンサーリンク
概要
- 論文の詳細を見る
A sex pheromone, PR-IP Inducer, which is released from mating-type minus cells of Closterium, was purified by monitoring its biological effect on the induction of the release of protoplast-release-inducing protein (PR-IP) from mating-type plus cells. The purified PR-IP Inducer had an apparent molecular mass of 22 kDa and of 18.7 kDa as determined by SDS-PAGE and mass-spectrometric analysis, respectively. Staining with periodic acid-Schiff reagent indicated that PR-IP Inducer included a glycan chain. From the analysis of a dose-response curve, it seemed that PR-IP Inducer was able to exert its activity over quite wide range of concentrations (1×10^<-10>-3×10^<-7> M). It appears that PR-IP Inducer is a novel glycoproteinaceous pheromone, as is PR-IP, and that it exerts its effect at the earliest stages of the sexual reproduction of the Closterium peracerosum-strigosum-littorale complex.
- 日本植物生理学会の論文
著者
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Fujii Tadashi
Institute of Biological Sciences, University of Tsukuba
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Nojiri T
Institute Of Biological Sciences University Of Tsukuba
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NOJIRI Takahiro
Institute of Biological Sciences, University of Tsukuba
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SEKIMOTO Hiroyuki
Institute of Biological Sciences, University of Tsukuba
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Fujii Tadashi
Institute Of Biological Sciences University Of Tsukuba
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Sekimoto Hiroyuki
Institute Of Biological Sciences University Of Tsukuba:recipent Of Fellowship For Japanese Junior Sc
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Fujii Tadashi
Institute Of Biological Science The University Of Tsukuba
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