Frequency Improvement of Somatic Embryogenesis at High Embryo Density by Partial Replacement of Medium in Carrot Suspension Cultures
スポンサーリンク
概要
- 論文の詳細を見る
The effects of medium replacement on the development of embryos from the globular to torpedo-shaped stage were investigated in carrot suspension cultures. Partial replacement of the medium was more effective than total or non-replacement at a high embryo density. The frequency of somatic embryogenesis (embryo development from globular to torpedo-shaped) at a high embryo density (500 globular embroys/l) was increased to 53.1% by partial replacement of the medium as compared to 36.8% without medium replacement. Partial replacement of the medium is a simple method that should prove useful for the mass production of somatic embryos.
- 社団法人日本生物工学会の論文
- 1997-09-25
著者
-
KAMADA Hiroshi
Gene Research Center, Graduate School of Life and Environmental Science, University of Tsukuba
-
Higashi K
Gene Research Center University Of Tsukuba
-
Higashi K
Gene Research Center Institute Of Biological Sciences University Of Tsukuba
-
Kamada H
Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
-
KOMAMINE Atsushi
Department of Chemical and Biological Sciences, Japan Women's University
-
Kamada Hiroshi
Gene Experiment Center University Of Tsukuba
-
Osuga Koichi
Advanced Tech.res.cent. Mitsubishi Heavy Ind.
-
Komamine A
The Research Institute Of Evolutionary Biology
-
Komamine Atsushi
Department Of Botany Faculty Of Science The University Of Tokyo:(present)biological Institute Facult
-
Kamada Hiroshi
Gene Exp. Center Univ. Tsukuba
関連論文
- Efficient transformation mediated by Agrobacterium tumefaciens with a ternary plasmid in Pharbitis nil
- Effect of Phytosulfokine-α on Growth and Tropane Alkaloid Production in Atropa belladonna Hairy Roots
- Identification of a Novel factor, Vanillyl Benzyl Ether, Which Inhibits Somatic Embryogenesis of Japanese Larch (Larix leptolepis Gordon)
- Production of Non-organogenic and Loosely Attached Callus in Leaf Disk Cultures of Haploid Nicotiana plumbaginifolia by N^ Ion Beam Irradiation
- Frequency of Mutations of the Transgene, which might Result in the Loss of the Glyphosate-Tolerant Phenotype, was Lowered in Roundup Ready^[○!R] Soybeans
- Phytosulfokine - α, a Peptidyl Plant Growth Factor, Stimulates Cell Cycle Progression in Carrot Non - Embryogenic Cells
- COOPERATIVE EFFECT OF PHYTOSULFOKINE (PSK) AND AUXIN ON CELL DIVISION OF CULTURED CARROT CELL
- A Secreted Peptide Growth Factor, Phytosulfokine, Acting as a Stimulatory Factor of Carrot Somatic Embryo Formation
- Promotive Effects of the Peptidyl Plant Growth Factor, Phytosulfokine-α, on the Growth and Chlorophyll Content of Arabidopsis Seedlings under High Night-time Temperature Conditions
- Constituents in Watercress : Inhibitors of Histamine Release from RBL-2H3 Cells Induced by Antigen Stimulation
- The Effect of Feeding Carrots on Immunoglobulin E Production and Anaphylactic Response in Mice
- Stimulatory Effects of a Novel Peptidyl Plant Growth Factor, Phytosulfokine-α, on the Adventitious Bud Formation from Callus of Antirrhinum majus
- Promotion by a Peptidyl Growth Factor, Phytosulfokine, of Chlorophyll Formation in Etiolated Cotyledon of Cucumber
- IAA Metabolism in Embryogenic and Non-Embryogenic Carrot Cells : GROWTH AND DEVELTOPMENT : PROTEINS, ENZYMES AND METABOLISM
- ANALYSIS OF THE GENES ENCODING PLASMA MEMBRANE AQUAPORINS IN CARROT (DAUCUS CAROTA) SOMATIC AND ZYGOTIC EMBRYOGENESIS
- Analysis of Sugars in Squash Xylem Sap
- On the participation of flowering and circadian clock-related genes to the photoperiodic induction of flowering in Pharbitis
- Structure-activity Relationship of Pamamycins : Effect of Side Chain Length on Aerial Mycelium-inducing Activity
- Isolation and structural properties of aerial mycelium differentiation-inhibitory substances against Streptomyces scabiei causing potato common scab
- P-72 BIOSYNTHETIC PATHWAY OF PAMAMYCIN, A NITROGEN-CONTAINING POLYKETIDE
- Anthranilic Acid, a Spore Germination Inhibitor of Phytopathogenic Streptomyces sp. B-9-1 Causing Root Tumor of Melon
- Purification from Conditioned Medium and Chemical Identification of a Factor That Inhibits Somatic Embryogenesis in Carrot
- Detection of Glycoproteins in Carrot Somatic Embryos Induced by 2,4-D or Stress Treatment
- EFFECTS OF CELL DENSITY ON CARROT SOMATICEMBRYOGENESIS
- Gibberellin Is Essentially Required for Carrot (Daucus carota L.) Somatic Embryogenesis : Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions(Biochemistry & Molecular Biology)
- Morphological and biochemical analysis of the mutants associated with intercellular attachment in haploid Nicotiana plumbaginifolia
- Deactivation of Gibberellin by 2-Oxidation during Germination of Photoblastic Lettuce Seeds(Organic Chemistry)
- REGULATION BY LIGHT AND LOCALIZATION OF GENE EXPRESSION OF GIBBERELLIN BIOSYNTHETIC ENZYMES DURING GERMINATION OF PHOTOBLASTIC LETTUCE SEEDS
- Regulation by Phytochrome of Gene Expression of Gibberellin Biosynthetic Enzymes during Germination of Photoblastic Lettuce Seeds
- Effects of the removal of cotyledons on endogenous gibberellin levels in hypocotyls of young cucumber and tomato seedlings
- INVOLVEMENT OF COTYLEDON AND GIBBERELLIN IN THE TISSUE-REUNION PROCESS OF CORTEX IN THE CUT HYPOCOTYL OF CUCUMBER AND TOMATO
- CLONING AND CHARACTERIZATION cDNAs ENCODING GIBBERELLIN BIOSYNTHETIC ENZYMES FROM LETTUCE
- Immediate induction of APETALA1-like gene expression following a single short-day in Pharbitis nil
- Isolation and characterization of novel genes controlled by short-day treatment in Pharbitis nil
- Isolation and analysis of a LEAFY homolog and TERMINAL FLOWER1 homologous from Pharbitis nil
- Characterization of Transcriptional Oscillation of an Arabidopsis Homolog of PnC401 Related to Photoperiodic Induction of Flowering in Pharbitis nil
- LOCALIZATION ANALYSIS OF mRNA AND PROTEINS OF CLOCK-CONTROLLED GENES (C401 AND GLP) RELATED TO PHOTOPERIODIC INDUCTION OF FLOWERING
- STRUCTURE AND FUNCTION OF A CLOCK-CONTROLLED GENE, ATC401,RELATED TO PHOTOPERIODIC INDUCTION OF FLOWERING
- Agrobacterium-Mediated Transformation and Regeneration of Pharbitis nil
- Isolation and Characterization of a MADS-box Gene cDNA, PnMADS1, That is Expressed in Both Vegetative and Floral Meristems of Pharbitis nil
- PROMOTER ANALYSIS OF CLOCK-CONTROLLED GENES (ATC401,PNC401) RELATING TO PHOTOPERIODIC INDUCTION OF FLOWERING
- FUNCTIONAL ANALYSIS OF A CLOCK-CONTROLLED GENE, GLP IN TRANSGENIC TOBACCO PLANTS
- REGULATION OF CLOCK-CONTROLLED GENES AND PHOTOPERIODIC INDUCTION OF FLOWERING
- CLONING, MAPPING AND EXPRESSION ANALYSIS OF TWO GENES OF GERMIN-LIKE PROTEIN IN Arabidopsis
- CLONING, MAPPING AND EXPRESSION ANALYSIS OF PNC401 HOMOLOGUE IN Arabidopsis thaliana
- EFFECT OF INORGANIC ELEMENTS ON THE TISSUE-REUNION PROCESS IN THE CUT CUCUMBER HYPOCOTYLS
- Comparison and Characterization of cis-Regulatory Regions in Some Embryo-Specific and ABA-Responsive Carrot Genes, DcECPs
- Dark-Induced Accumulation of mRNA for a Homolog of Translationally Controlled Tumor Protein (TCTP) in Pharbitis
- Transient Increase in the Level of mRNA for a Germin-Like Protein in Leaves of the Short-Day Plant Pharbitis nil during the Photoperiodic Induction of Flowering
- Isolation and Characterization of Six Abscisic Acid-Inducible Genes from Carrot Somatic Embryos
- INTERACTIVE ACTION BETWEEN AUXIN AND PSK-α ON CELL DIVISION OF CARROT NON-EMBRYOGENIC CELLS
- THE EFFECTS OF PSK-α ON GENE EXPRESSION OF CELL CYCLE-RELATED GENES IN CARROT CELL CULTURE
- Analysis of cis-Regulatory Elements in Carrot Embryo-Specific and ABA-Responsive Gene, DcECP31
- Structure of a New Opine, Mikimopine, in Hairy Root Induced by Agrobacterium rhizogenes(Organic Chemistry)
- POVERTY OF METHYLESTERIFIED AND PERIPHERALLY LOCATED PECTINS IN LOOSELY ATTACHED NON-EMBRYOGENIC CALLUS OF CARROT
- EXPRESSION ANALYSIS OF EMBRYOGENESIS RELATED GENES USING IN SITU HYBRIDIZATION
- ISOLATION AND CHARACTERIZATION OF PIF3 GENES FROM HORSERADISH
- MOLECULAR CLONING OF cDNAs SPECIFICALLY EXPRESSED DURING INDUCTIVE PHOTOPERIOD OF FLOWERING BY DIFFERENTIAL DISPLAY IN Pharbitis nil
- CDNA CLONING AND CHARACTERIZATION OF THE Pharbitis nil FLORICAURA AND LEAFY HOMOLOG
- Molecular Identification of Two Genes of Germin-like Protein in Arabidopsis
- Phytochrome Activities and Biological Activities of Genes for Phytochrome A of Horseradish in Horseradish Hairy Roots
- Calcium-dependent protein phosphorylation in hairy roots of Daucus carota
- CALCIUM-DEPENDENT PROTEIN PHOSPHORYLATION IN HAIRY ROOTS OF Daucus carota
- Efficacy of a special screened greenhouse covered by duplex fine mesh in reducing maize outcrossing
- Effect of a special screened greenhouse covered by fine mesh on maize outcrossing
- Reducing gene flow from pollen dispersal of genetically modified plants in special screened greenhouses
- Xylem Sap Lectin, XSP30, Recognizes GlcNAc Sugar Chains of Glycoproteins in Cucumber Leaves
- Cysteine Proteinase Gene Expression in the Endosperm of Germinating Carrot Seeds(Biochemistry & Molecular Biology)
- Cloning of a Full-length cDNA Encoding ent-Kaurene Synthase from Gibberella fujikuroi : Functional Analysis of a Bifunctional Diterpene Cyclase
- Changes in Protein Pattern during Stress-induction of Carrot (Daucus carota L.) Somatic Embryogenesis
- ISOLATION AND CHARACTERIZATION OF PHYTOCHROME GENES FROM HORSERADISH HAIRY ROOTS ・II : PHYB AND PHYE GENES
- ISOLATION AND CHARACTERIZATION OF PHYTOCHROME GENES FROM HORSERADISH HAIRY ROOTS
- PRESERVATION OF HAIRY ROOTS AT LOW TEMPERATURES
- Inhibitory Factor(s) of Somatic Embryogenesis Regulated Suspensor Differentiation in Suspension Culture of Japanese Larch (Larix leptolepis GORDON)
- An in vitro Culture System used to Investigate Possible Interactions between the Embryo Proper and the Suspensor in Embryogenesis in Japanese Larch (Larix leptolepis GORDON)
- Inhibitions of Growth and Lateral Branch Development by Calmodulin Antagonists in Hairy Roots of Lithospermum erythrorhizon, Atropa belladonna and Daucus carota
- Stimulatory and Inhibitory Conditioning Factors that Regulate Cell Proliferation and Morphogenesis in Plant Cell Cultures
- C-ABI3, the Carrot Homologue of the Arabidopsis ABI3, is Expressed during Both Zygotic and Somatic Embryogenesis and Functions in the Regulation of Embryo-Specific ABA-Inducible Genes
- Frequency Improvement of Somatic Embryogenesis at High Embryo Density by Partial Replacement of Medium in Carrot Suspension Cultures
- EFFECTS OF CONDITIONING FACTOR(S) ON SOMATIC EMBRYOGENESIS IN CARROT SUSPENSION CULTURES
- Inhibitory Effects of p-Hydroxybenzyl Alcohol on Somatic Embryogenesis in Carrot Cell Cultures
- Environmental risk assessment of genetically modified chrysanthemums containing a modified cry1Ab gene from Bacillus thuringiensis
- Genetic Evidence for the Role of Isopentenyl Diphosphate Isomerases in the Mevalonate Pathway and Plant Development in Arabidopsis
- SUSPENSOR REGULATES DEVELOPEMET OF EMBRYO PROPER DURING THE EARLY SOMATIC EMBRYOGENESIS IN JAPANEASE LARCH (Larix leptolepis GORDON)
- The Promotive Effects of a Peptidyl Plant Growth Factor, Phytosulfokine-α, on the Formation of Adventitious Roots and Expression of a Gene for a Root-Specific Cystatin in Cucumber Hypocotyls
- FUNCTION ANALYSIS OF C-AB13 IN TRANSGENIC CARROT NON-EMBRYOGENIC CELLS
- Effects of High and Very Low Magnetic Fields on the Growth of Hairy Roots of Daucus carota and Atropa belladonna
- Gene literacy education in Japan-Fostering public understanding through practice of hands-on laboratory activities in high schools
- Arabidopsis CYP85A2 Catalyzes Lactonization Reactions in the Biosynthesis of 2-Deoxy-7-oxalactone Brassinosteroids
- Induction of double flowers in Pharbitis nil using a class-C MADS-box transcription factor with Chimeric REpressor gene-Silencing Technology
- Enzymatic Total Synthesis of Gibberellin A_4 from Acetate
- Involvement of the CYP78A Subfamily of Cytochrome P450 Monooxygenases in Protonema Growth and Gametophore Formation in the Moss Physcomitrella patens
- Isolation and Characterization of a Spore Germination Inhibitor from Streptomyces sp. CB-1-1, a Phytopathogen Causing Root Tumor of Melon
- Evaluation of gene flow and its environmental effects in the field
- Optical isomers of abscisic acid in carrot somatic embryos have the same effect on induction of dormancy and desiccation tolerance
- Induction of double flowers in Pharbitis nil using a class-C MADS-box transcription factor with Chimeric REpressor gene-Silencing Technology
- Functional Identification of a Rice ent-Kaurene Oxidase, OsKO2, Using the Pichia pastoris Expression System
- Efficient transformation mediated by Agrobacterium tumefaciens with a ternary plasmid in Pharbitis nil
- Constitutive expression of the GIGANTEA Ortholog Affects Circadian Rhythms and Suppresses One-shot Induction of Flowering in Pharbitis nil, a Typical Short-day Plant
- Effect of Pamamycin-607 on Secondary Metabolite Production by Streptomyces spp.