Optical isomers of abscisic acid in carrot somatic embryos have the same effect on induction of dormancy and desiccation tolerance
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概要
- 論文の詳細を見る
Carrot (Daucus carota) somatic embryos have been extensively used as an experimental system for studying plant embryogenesis. In maturing zygotic embryos, the phytohormone abscisic acid (ABA) is involved in induction of dormancy and desiccation tolerance. Somatic embryos contain only low levels of endogenous ABA and lack desiccation tolerance and dormancy, but both tolerance and dormancy can be induced by application of exogenous ABA. We analyzed the effects of the optical isomers of ABA, (S)-(+)-2-cis-ABA [(+)-ABA] and (R)-(−)-2-cis-ABA [(−)-ABA], and a racemic mixture of these two isomers (racemic ABA) on the carrot system. Somatic embryos treated with (+)-ABA, (−)-ABA, or racemic ABA showed the same levels of growth inhibition and desiccation tolerance. Similarly, each ABA isomer and racemic ABA had the same effect on accumulation of transcripts for ABA-inducible genes. The results indicate that both (+)-ABA and (−)-ABA cause the same amount of activity in these physiological phenomena. Our findings suggest that strict steric requirements of the ABA signaling pathways are absent in carrot somatic embryos, and we propose that commercially available racemic ABA is as effective as the natural isomer (+)-ABA in induction of dormancy and desiccation tolerance in carrot somatic embryos.
- 日本植物細胞分子生物学会の論文
- 2008-12-01
著者
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KAMADA Hiroshi
Gene Research Center, Graduate School of Life and Environmental Science, University of Tsukuba
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SHIOTA Hajime
International Graduate School of Arts and Sciences, Yokohama City University
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Kamada Hiroshi
Gene Experiment Center University Of Tsukuba
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Shiota Hajime
International Graduate School Of Arts And Sciences Yokohama City Univ.
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Shiota Hajime
International Graduate School Of Arts And Sciences Yokohama City University
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Kamada Hiroshi
Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Kamada Hiroshi
Gene Exp. Center Univ. Tsukuba
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