Isolation of novel gain- and loss-of-function alleles of the circadian clock gene LATE ELONGATED HYPOCOTYL (LHY) in Arabidopsis
スポンサーリンク
概要
- 論文の詳細を見る
In Arabidopsis, two myb-related proteins, LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), play key roles in the control of circadian rhythms. Photoperiodic flowering and elongation of hypocotyls are regulated by a circadian clock. Here, we report the isolation and characterization of a novel deletion allele (lhy-14) and a gain-of-function allele (lhy-2) of lhy. Based on long hypocotyl and late flowering phenotypes under long days, lhy-2 was isolated as an intragenic suppressor of lhy-12, one of the loss-of-function alleles of lhy. Although the lhy-12 mRNA has a 19-bp truncation, we found that this was not caused by a deletion in the LHY but by a point mutation. We propose a model explaining the loss-of-function of LHY in lhy-12 and the partial suppression of the lhy-12 phenotype by lhy-2, by the abnormal splicing of LHY mRNA.
- 日本植物細胞分子生物学会の論文
- 2007-12-01
著者
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KAMADA Hiroshi
Institute of Biological Sciences, University of Tsukuba
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Coupland George
Max Planck Institute For Plant Breeding
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Mizoguchi Tsuyoshi
Gene Res. Center Univ. Of Tsukuba
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Mizoguchi Tsuyoshi
Institute Of Biological Sciences University Of Tsukuba
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Kamada Hiroshi
Univ. Tsukuba Ibaraki Jpn
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Oda Atsushi
Institute Of Biological Sciences University Of Tsukuba
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Kamada Hiroshi
Institute Of Biological Sciences Univ.
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REEVES Paul
Max Planck Institute for Plant Breeding
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TAJIMA Takeomi
Institute of Biological Sciences, University of Tsukuba
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NAKAGAWA Mayu
Institute of Biological Sciences, University of Tsukuba
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Nakagawa Mayu
Gene Research Center, University of Tsukuba
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Oda Atsushi
Gene Research Center, University of Tsukuba
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Oda Atsushi
Gene Research Center University Of Tsukuba
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Nakagawa Mayu
Gene Research Center University Of Tsukuba
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TAJIMA Takeomi
Gene Research Center, University of Tsukuba
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Mizoguchi Tsuyoshi
Institute Of Biological Sciences Tsukuba University
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Kamada Hiroshi
Institute Of Biological Sciences Tsukuba University
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