Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens
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概要
- 論文の詳細を見る
Sequencing a whole genome, cataloguing full-length cDNAs and ESTs, and construction of a comprehensive mutant population are essential steps in genome projects of individual model species. Tomato is one of the most important model crops to be undertaken for study over the next few decades, and the Solanaceae Genomics Project (SOL) has begun genome projects as international collaborations. The Solanum lycopersicum cultivar Micro-Tom shares several advantages with Arabidopsis, including its small size, short life cycle (approximately 90 days), and growth normally under artificial light. Here we demonstrate an initial attempt to generate a comprehensive mutant population in Micro-Tom. A total of 3,839 M2 families derived from ethylmethanesulfonate (EMS) mutagenesis were visually phenotyped, and putative mutants were classified into 15 primary and 48 secondary categories based on the SOL database, “The Genes That Make Tomatoes”.
- 日本植物細胞分子生物学会の論文
- 2007-03-01
著者
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Aoki Koh
Kazusa DNA Research Institute
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江面 浩
筑波大学大学院生命環境科学研究科遺伝子実験センター
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江面 浩
筑波大学大学院生命環境科学研究科生物圏資源科学専攻
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SHIBATA Daisuke
Kazusa DNA Research Institute
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Mori Hitoshi
Graduate School of Bioagricultural Sciences, Nagoya University
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江面 浩
筑波大 大学院生命環境科学研究科 遺伝子実験セ
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Mori Hitoshi
Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Ezura Hiroshi
Graduate School Of Life And Environmental Sci. Univ. Of Tsukuba
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Ezura Hiroshi
Gene Research Center University Of Tsukuba
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Yamazaki Yukiko
Center For Genetic Resource Information National Institute Of Genetics
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Ezura Hiroshi
Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba
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溝口 剛
筑波大院生命環境科学研究科
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IMANISHI Shunsuke
National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
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Imanishi Shunsuke
National Institute Of Vegetable And Tea Science Naro
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Mizoguchi Tsuyoshi
Gene Res. Center Univ. Of Tsukuba
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Mizoguchi Tsuyoshi
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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江面 浩
筑波大学大学院生命環境科学研究科
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WATANABE Shin
Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
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Aoki Koh
Kazusa Dna Res. Inst. Chiba Jpn
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Kubo Yasutaka
Graduate School Of National Science Okayama University
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WATANABE Shin
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Shibata Daisuke
Kazusa Dna Res. Inst. Chiba Jpn
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Shibata Daisuke
Kazusa Dna Res. Inst.
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Aoki Koh
Kazusa Dna Res. Institutes
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Mori Hitoshi
Graduate School Of Bio-agricultural Sciences Nagoya University
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Watanabe Shin
Gene Research Center University Of Tsukuba
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Imanishi Shunsuke
National Inst. Of Vegetable And Tea Sci. National Agriculture And Food Res. Organization
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