Improvement of the quantitative differential metabolome pipeline for gas chromatography-mass spectrometry data by automated reliable peak selection
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概要
- 論文の詳細を見る
Recent advances in metabolomics technology have enabled large-scale comprehensive analyses of metabolites, but the throughput of data processing of non-targeted, quantitative differential analyses is very low. It is crucial to solve this problem to generate biological hypotheses from a large-scale dataset. To improve the analysis of metabolite data, we focused on the processing of quantitative differential analysis after multiple peak alignment. We have developed a program named FAQuant that automatically selects reliable peaks from each chromatogram, quantifies the mean of peak intensity to compare between sample groups, and selects the peaks with differences in accumulation of metabolites. This program was incorporated into a quantitative differential metabolome pipeline as a module to improve the throughput of gas chromatography-mass spectrometry dataset analysis. As a result, the module incorporation largely reduced the total processing time. Furthermore, differential analysis of metabolites in soybean (Glycine max) cultivars was demonstrated by use of the system. This system might facilitate biological hypothesis generation from large-scale comparative metabolome analysis.
- 日本植物細胞分子生物学会の論文
- 2009-12-25
著者
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Ara Takeshi
Kazusa DNA Research Institute
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Aoki Koh
Kazusa DNA Research Institute
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Suzuki Hideyuki
Kazusa DNA Research Institute
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Sasaki Kanako
Research Institute For Sustainable Humanosphere Kyoto University
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SHIBATA Daisuke
Kazusa DNA Research Institute
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Sakurai Nozomu
Kazusa DNA Research Institute
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Suzuki Kaoru
Institute For Biological Resources And Functions Aist
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Ashikari T
Nihon Univ. Kanagawa Jpn
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Aoki T
Nihon Univ. Kanagawa Jpn
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Shinshi H
Kazusa Dna Research Institute
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TANGE Yoshie
Kazusa DNA Research Institute
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MORISHITA Yoshihiko
Kazusa DNA Research Institute
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SAITO Kazuki
Kazusa DNA Research Institute
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Shibata Daisuke
Graduate School Of Life Sciences Tohoku University
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Seki Hikaru
Plant Sci. Center Riken
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Shimada Norimoto
Department Of Applied Biological Sciences Nihon University
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Sakakibara Norikazu
Institute For Sustainable Humanosphere Kyoto University
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Aoki Koh
Kazusa Dna Res. Inst. Chiba Jpn
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Shibata D
Graduate School Of Life Sciences Tohoku University
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Saito Kazuki
Chiba Univ. Chiba Jpn
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Shimada Norimoto
Department Of Life Science Aichi University Of Education
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Shibata Daisuke
Kazusa Dna Res. Inst.
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Aoki Koh
Kazusa Dna Res. Institutes
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Aoki Toshio
Department Of Applied Biological Sciences Nihon University
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Shibata Daisuke
Mitsui Plant Biotech. Res. Inst.
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Shinshi Hideaki
Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.
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Suzuki Kaoru
Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.
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Ara Takeshi
Kazusa Dna Res. Inst.
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青木 考
かずさDNA研:大阪府大
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