Establishment of the evaluation system of salt tolerance on transgenic woody plants in the special netted-house
スポンサーリンク
概要
- 論文の詳細を見る
Salinity is regarded as a major factor impacting the growth of plants such as trees. Transgenic technology is considered an effective approach to minimizing salt damage by enhancing a plants salt tolerance. Currently, most of the research on transgenic woody plants is still limited to the laboratory level in vitro and in growth chambers. However, the confined laboratory evaluation of salt tolerance condition yields minimal information since various factors that significantly impact growth, such as weather and season, do not come into play. Field level evaluation of salt tolerance is necessary but is limited in Japan. Transgenic plants should be evaluated in semi-confinement before they are moved to open fields. Therefore, an effective evaluation method for salt tolerance in woody plants is needed in semi-confinement. To evaluate the salt tolerance of transgenic Eucalyptus under semi-confined conditions, we initially developed a new evaluation method using non-transgenic E. camaldulensis. The duration of salt treatment was determined to be the point in time when half of the plants started to exhibit wilting. Additionally, the plants were continuously irrigated with fresh water as a final procedure. With the evaluation system in place, the salt tolerance of transgenic E. globulus containing the choline oxidase (codA) gene was measured. The transgenic plants showed higher survival rates than the non-transgenic plants. This result indicated that the salt tolerance of transgenic E. globulus was enhanced by the introduced codA gene and also established that the new evaluation system is valuable for testing transgenic plants in semi-confinement and will contribute to an increase in the useful applications of transgenic plants.
- 2009-03-25
著者
-
Suzuki Hideyuki
Kazusa DNA Research Institute
-
KIKUCHI Akira
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
MATSUNAGA Etsuko
Forest Science Laboratory, Nippon Paper Industries Co., Ltd.
-
NANTO Kazuya
Forest Science Laboratory, Nippon Paper Industries Co., Ltd.
-
SHIMADA Teruhisa
Forest Science Laboratory, Nippon Paper Industries Co., Ltd.
-
WATANABE Kazuo
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
SHIBATA Daisuke
Kazusa DNA Research Institute
-
Sakurai Nozomu
Kazusa DNA Research Institute
-
Kikuchi Akira
Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
-
Nanto Kazuya
Forestry Science Laboratory Nippon Paper Industries Co. Ltd
-
YU Xiang
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
Kikuchi Akira
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Shinshi H
Kazusa Dna Research Institute
-
MORISHITA Yoshihiko
Kazusa DNA Research Institute
-
Shibata Daisuke
Graduate School Of Life Sciences Tohoku University
-
Shimada Teruhisa
Forestry Science Laboratory Nippon Paper Industries Co. Ltd
-
Matsunaga Etsuko
Forestry Science Laboratory Nippon Paper Industries Co. Ltd
-
Shimazaki Takayoshi
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Seki Hikaru
Plant Sci. Center Riken
-
Yu Xiang
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Shimada Norimoto
Department Of Applied Biological Sciences Nihon University
-
Sakakibara Norikazu
Institute For Sustainable Humanosphere Kyoto University
-
Shibata D
Graduate School Of Life Sciences Tohoku University
-
Watanabe Kazuo
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Shimada Norimoto
Department Of Life Science Aichi University Of Education
-
Shibata Daisuke
Kazusa Dna Res. Inst. Chiba Jpn
-
Shibata Daisuke
Kazusa Dna Res. Inst.
-
Shibata Daisuke
Mitsui Plant Biotech. Res. Inst.
-
Shinshi Hideaki
Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.
-
Watanabe Kazuo
Graduate School Of Biosphere Sciences Hiroshima University
-
Watanabe Kazuo
Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
関連論文
- Transient increase in salicylic acid and its glucose conjugates after wounding in Arabidopsis leaves
- The "all-in-one" rol-type binary vectors as a tool for functional genomic studies using hairy roots
- KATANA: A web-based guide to public databases for Arabidopsis genomic information
- Allelopathy assessments for the environmental biosafety of the salt-tolerant transgenic Eucalyptus camaldulensis, genotypes codA 12-5B, codA 12-5C, and codA 20C
- Establishment of a homogenized method for environmental biosafety assessments of transgenic plants
- Suppression of carotenoid synthesis in transgenic Arabidopsis cultured cells over-expressing the AHL29/SOB3 gene
- Estimation for Different Genotypes of Plants based on DNA Analysis using Near-infrared (NIR) and Fourier-transform Infrared (FT-IR) Spectroscopy
- Use of Rice SSR Markers as RAPD Markers for Genetic Diversity Analysis in Zingiberaceae
- An approach to peak detection in GC-MS chromatograms and application of KNApSAcK database in prediction of candidate metabolites
- In vitro proliferation and triterpenoid characteristics of licorice (Glycyrrhiza uralensis Fischer, Leguminosae) stolons
- Transient increase in salicylic acid and its glucose conjugates after wounding in Arabidopsis leaves
- P-551 FUNCTIONS OF ARABIDOPSIS CAldOMT
- Occurrence of guaiacyl/p-hydroxyphenyl lignin in Arabidopsis thaliana T87 cells
- Mycorrhizal colonization of transgenic Eucalyptus camaldulensis carrying the mangrin gene for salt tolerance
- Mycorrhizal colonization of transgenic Eucalyptus camaldulensis carrying the mangrin gene for salt tolerance
- P-392 BIOSYNTHETIC PATHWAY OF PHYTOSTEROLS VIA LANOSTEROL
- Functional Analysis of Isoforms of NADPH:Protochlorophyllide Oxidoreductase (POR), PORB and PORC, in Arabidopsis thaliana
- At5g54160 gene encodes Arabidopsis thaliana 5-hydroxyconiferaldehyde O-methyltransferase
- Simple functional analysis of key genes involved in astaxanthin biosynthesis using Arabidopsis cultured cells
- Catalog of Micro-Tom tomato responses to common fungal, bacterial, and viral pathogens
- Expressed sequence tags from rhizomes of Glycyrrhiza uralensis
- Meatabolomic analysis of model legume plant Lotus japonicus
- Establishment of the evaluation system of salt tolerance on transgenic woody plants in the special netted-house
- An Arabidopsis Sigma Factor (SIG2)-Dependent Expression of Plastid-Encoded tRNAs in Chloroplasts
- ANALYSIS OF COMPONENTS OF THE PLASTID PROTEIN IMPORT APPARATUS IN ARABIDOPSIS
- Global political, economic, social and technological issues on trasngenic crops
- Microscale alkaline nitrobenzene oxidation method for high-throughput determination of lignin aromatic components
- Microscale alkaline nitrobenzene oxidation method for high-throughput determination of lignin aromatic components
- Two routes of steroidal backbone synthesis in plants
- GENOME WIDE EXPRESSION MONITORING OF JASMONATE-RESPONSIVE GENES OF ARABIDOPSIS USING cDNA MACROARRAY REVEALED SELF-ACTIVATION OF A SERIES OF JASMONATE BIOSYNTHESIS GENES AND CRPSS TALK WITH OTHER SIGNALING PATHWAYS
- GENOME-WIDE EXPRESSION MONITORING OF JASMONATE-RESPONSIVE GENES OF ARABIDOPSIS USING A cDNA ARRAY
- DNA array technology and gene expression analysis in Arabidopsis thaliana
- A gene co-expression database for understanding biological processes in soybean
- A database for poplar gene co-expression analysis for systematic understanding of biological processes, including stress responses (Special issue on wood science and technology for mitigation of global warming)
- Over-expression of transcription associated factor genes co-expressed with genes of the mevalonate pathway, upstream of isoprenoid biosynthesis, in Arabidopsis cultured cells
- Approaches for Extracting Practical Information from Gene Co-expression Networks in Plant Biology
- Role of the Putative Membrane-Bound Endo-1,4-β-Glucanase KORRIGAN in Cell Elongation and Cellulose Synthesis in Arabidopsis thaliana
- A MEMBRANE-BOUND ENDO-1,4-β-GLUCANASE IS REQUIRED FOR CELLULOSE BIOSYNTHESIS IN ARABIDOPSIS
- Improvement of the quantitative differential metabolome pipeline for gas chromatography-mass spectrometry data by automated reliable peak selection
- A batch processing protocol for construction of expression vector plasmids from a cDNA collection and Agrobacterium-mediated transformation of suspension-cultured cells of Arabidopsis
- Metabolomics for the characterization of cytochromes P450-dependent fatty acid hydroxylation reactions in Arabidopsis
- ARTRA : a new database of the Arabidopsis transcriptome and gene-specific sequences for microarray probes and RNAi triggers
- Functional genomics of the Dof transcription factor family genes in suspension-cultured cells of Arabidopsis thaliana
- DAGViz : a directed acyclic graph browser that supports analysis of Gene Ontology annotation
- A tool for high-throughput prediction of molecular formulas and identification of isotopic peaks from large-scale mass spectrometry data
- Analysis of group II ERF family genes in Arabidopsis thaliana
- P-497 PLANT LANOSTEROL SYNTHASE
- Plant Lanosterol Synthase : Divergence of the Sterol and Triterpene Biosynthetic Pathways in Eukaryotes
- Studies on Transcriptional Regulation of Endogenous Genes by ERF2 Transcription Factor in Tobacco Cells
- ISOLATION OF VACUOLAR MEMBRANE FROM Arabidopsis AND IDENTIFICATION OF ITS PROTEINS
- Metabolite Profiling of Chalcones and Flavanones in Tomato Fruit
- Dikaryotic arthroconidiation of Pleurotus subgenus Coremiopleurotus
- The Arabidopsis DREB1A gene driven by the stress-inducible rd29A promoter increases salt-stress tolerance in proportion to its copy number in tetrasomic tetraploid potato (Solanum tuberosum)
- Isolation and Characterization of Sea Aster Salt-Stress Responsive Cysteine Protease Gene Obtained by a Hetero-probed Macroarray
- REGULATORY MECHANISM OF CYCLOHEXIMIDE-RESPONSIVE EXPRESSION OF ERF3 GENE IN TOBACCO
- Down-Regulation of Cyclin Genes Induced by Fungal Elicitors in Tobacco Cells
- Studies on Regulatory Mechanism of Wound-Inducible Expression of ERF3 Gene in Tobacco Plants
- Expression Patterns of Elicitor-Responsive Genes in Tobacco Plants
- Analysis of Regulatory Mechanism of Wound-Responsive Expression of the Tobacco ERF Genes
- STUDIES ONT HE MECHANISM OF CELL DEATH INDUCED BY XYLANASE IN TOBACCO SUSPENSION CULTURE
- MECHANISM OF ELICITOR-INDUCED CELL DEATH IN TOBACCO CELL CULTURE
- Effect of the WUS (JAM) gene on the regulation of leaf identity in Arabidopsis thaliana
- ANALYSIS OF AUTOPHAGY IN HIGHER PLANTS
- Genetic engineering of glycine betaine biosynthesis to enhance abiotic stress tolerance in plants
- Gene Expression Profiling of Coccolith-Bearing Cells and Naked Cells in Haptophyte Pleurochrysis haptonemofera with a cDNA Macroarray System
- Expressed sequence tags of full-length cDNA clones from the miniature tomato (Lycopersicon esculentum) cultivar Micro-Tom
- Assessment of Genetic Diversity among the Major Myanmar Banana Landraces
- Tolerance to Salt Stress of the Transgenic Tetrasomic Tetraploid Potato, Solanum tuberosum cv. Desiree Appears to be Induced by the DREB1A Gene and rd29A Promoter of Arabidopsis thaliana
- Construction of Integrated Genetic Map between Various Existing DNA Markers and Newly Developed P450-related PBA Markers in Diploid Potato (Solanum tuberosum)
- 2-Hydroxyisoflavanone Dehydratase is a Critical Determinant of Isoflavone Productivity in Hairy Root Cultures of Lotus japonicus
- Biosafety system frameworks for living modified organisms in Japan and Taiwan
- Experience on regulatory procedures and field assessment of genetically engineered Eucalyptus
- Characterization of Gene Expression of NsERFs, Transcription Factors of Basic PR Genes from Nicotiana sylvestris
- KAGIANA : An Excel-Based Tool for Retrieving Summary Information on Arabidopsis Genes
- Efficient and High-Throughput Vector Construction and Agrobacterium-Mediated Transformation of Arabidopsis thaliana Suspension-Cultured Cells for Functional Genomics
- Relationships of Zingiber species, and genetic variability assessment in ginger (Zingiber officinale) accessions from ex-situ genebank, on-farm and rural markets
- Impact of Environmental Stress-Tolerant Transgenic Potato on Genotypic Diversity of Microbial Communities and Soil Enzyme Activities under Stress Conditions
- Structure and expression of EREBP genes that regulate tobacco basic PR genes
- A database for poplar gene co-expression analysis for systematic understanding of biological processes, including stress responses
- Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin
- Global political, economic, social and technological issues on trasngenic crops
- KATANA : A web-based guide to public databases for Arabidopsis genomic information
- Plant expressed sequence tags databases: practical uses and the improvement of their searches using network module analysis
- Occurrence of guaiacyl/p-hydroxyphenyl lignin in Arabidopsis thaliana T87 cells
- Analysis of expressed sequence tags in developing secondary xylem and shoot of Acacia mangium
- Isolation of putative embryo-specific genes using stress induction of carrot somatic embryos
- Sugar signaling is involved in histone deacetylase-mediated repression of embryonic characteristics after germination
- Contribution of HDACs in the regulation of phase transition from embryogenesis to vegetative growth
- Plant expressed sequence tags databases : practical uses and the improvement of their searches using network module analysis
- MatchedlonsFinder : A software tool for revising alignment matrices of spectrograms from liquid chromatography-mass spectrometry
- High-Throughput Cryopreservation of Plant Cell Cultures for Functional Genomics
- Development of transgenic plants in jatropha with drought tolerance
- Dynamic metabolic changes during fruit maturation in Jatropha curcas L.
- Development of KaPPA-View4 for omics studies on Jatropha and a database system KaPPA-Loader for construction of local omics databases
- 2Fa06 KOMICS : metabolomics databases and tools for biotechnology(Advances in PhytoGenomics)
- ATL54, a RING-H2 domain protein selected by a gene co-expression network analysis, is associated with secondary cell wall formation in Arabidopsis
- Long-Chain Free Fatty Acid Profiling Analysis by Liquid Chromatography–Mass Spectrometry in Mouse Treated with Peroxisome Proliferator-Activated Receptor α Agonist
- Arabidopsis ABCB21 is a Facultative Auxin Importer/Exporter Regulated by Cytoplasmic Auxin Concentration
- Substrate-enantiomer selectivity of matairesinol O-methyltransferases
- ATL54, a RING-H2 domain protein selected by a gene co-expression network analysis, is associated with secondary cell wall formation in Arabidopsis