Metabolomics for the characterization of cytochromes P450-dependent fatty acid hydroxylation reactions in Arabidopsis

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概要

• 論文の詳細を見る

• Genome sequence analysis has revealed the presence of almost infinite numbers of cytochrome P450 genes in a variety of organisms. To establish a robust experimental platform from which to explore the catalytic potential of those putative P450 proteins, we have developed a comprehensive metabolic profiling system based on Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR/MS) analysis, together with recombinant enzyme studies. Here, we report the enzymatic properties of CYP78A5, CYP78A7, CYP78A10, and CYP86C3 of Arabidopsis as short-chain fatty acid hydroxylases, with substrates including lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), and myristoleic acid (C14:1). The fatty acid hydroxylation activity of CYP78A7 was confirmed using FT-ICR/MS metabolic profiling of P450-gene-overexpressing lines of cultured Arabidopsis T87 cells. The practical application of this metabolic profiling scheme is discussed as a P450 functional characterization platform.

• 日本植物細胞分子生物学会の論文
• 2009-03-25

著者

• Suzuki Hideyuki

  Kazusa DNA Research Institute

• SHIBATA Daisuke

  Kazusa DNA Research Institute

• Sakurai Nozomu

  Kazusa DNA Research Institute

• Shinshi H

  Kazusa Dna Research Institute

• KAI Kosuke

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• HASHIDZUME Hiroko

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• YOSHIMURA Kazuya

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• OHTA Daisaku

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• Shibata Daisuke

  Graduate School Of Life Sciences Tohoku University

• Seki Hikaru

  Plant Sci. Center Riken

• Shimada Norimoto

  Department Of Applied Biological Sciences Nihon University

• Ohta Daisaku

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Sakakibara Norikazu

  Institute For Sustainable Humanosphere Kyoto University

• Hashidzume Hiroko

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Kai Kosuke

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Shibata D

  Graduate School Of Life Sciences Tohoku University

• Yoshimura Kazuya

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Shimada Norimoto

  Department Of Life Science Aichi University Of Education

• Shibata Daisuke

  Kazusa Dna Res. Inst.

• Shibata Daisuke

  Mitsui Plant Biotech. Res. Inst.

• Shinshi Hideaki

  Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.

• Ohta Daisaku

  Graduate School Of Life And Environmental Sciences Osaka Prefecture Univ.

• Kai Kosuke

  Graduate School Of Life And Environmental Sciences Osaka Prefecture Univ.

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