Metabolomics for the characterization of cytochromes P450-dependent fatty acid hydroxylation reactions in Arabidopsis
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概要
- 論文の詳細を見る
Genome sequence analysis has revealed the presence of almost infinite numbers of cytochrome P450 genes in a variety of organisms. To establish a robust experimental platform from which to explore the catalytic potential of those putative P450 proteins, we have developed a comprehensive metabolic profiling system based on Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR/MS) analysis, together with recombinant enzyme studies. Here, we report the enzymatic properties of CYP78A5, CYP78A7, CYP78A10, and CYP86C3 of Arabidopsis as short-chain fatty acid hydroxylases, with substrates including lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), and myristoleic acid (C14:1). The fatty acid hydroxylation activity of CYP78A7 was confirmed using FT-ICR/MS metabolic profiling of P450-gene-overexpressing lines of cultured Arabidopsis T87 cells. The practical application of this metabolic profiling scheme is discussed as a P450 functional characterization platform.
- 日本植物細胞分子生物学会の論文
- 2009-03-25
著者
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Suzuki Hideyuki
Kazusa DNA Research Institute
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SHIBATA Daisuke
Kazusa DNA Research Institute
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Sakurai Nozomu
Kazusa DNA Research Institute
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Shinshi H
Kazusa Dna Research Institute
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KAI Kosuke
Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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HASHIDZUME Hiroko
Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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YOSHIMURA Kazuya
Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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OHTA Daisaku
Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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Shibata Daisuke
Graduate School Of Life Sciences Tohoku University
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Seki Hikaru
Plant Sci. Center Riken
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Shimada Norimoto
Department Of Applied Biological Sciences Nihon University
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Ohta Daisaku
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Sakakibara Norikazu
Institute For Sustainable Humanosphere Kyoto University
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Hashidzume Hiroko
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Kai Kosuke
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Shibata D
Graduate School Of Life Sciences Tohoku University
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Yoshimura Kazuya
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Shimada Norimoto
Department Of Life Science Aichi University Of Education
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Shibata Daisuke
Kazusa Dna Res. Inst.
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Shibata Daisuke
Mitsui Plant Biotech. Res. Inst.
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Shinshi Hideaki
Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.
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Ohta Daisaku
Graduate School Of Life And Environmental Sciences Osaka Prefecture Univ.
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Kai Kosuke
Graduate School Of Life And Environmental Sciences Osaka Prefecture Univ.
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