The "all-in-one" rol-type binary vectors as a tool for functional genomic studies using hairy roots
スポンサーリンク
概要
- 論文の詳細を見る
The hairy root culture system has several desirable features as an experimental model for functional analyses of the genes involved in root metabolism and/or physiology. We developed a binary vector set for efficient target gene overexpression and RNA interference (RNAi) in transformed (hairy) roots. The vectors, pHR-OX and pHR-RNAi, contain a cluster of rol (rooting locus) genes, together with the single GATEWAYTM conversion cassette (in pHR-OX vectors) or inverted repeats of the GATEWAYTM conversion cassette separated by an intron sequence (in pHR-RNAi vectors), flanked by a dual CaMV 35S promoter and nopaline synthase polyadenylation signal, on the same T-DNA. Transformation experiments with pHR-OX vectors using Arabidopsis, potato, and tobacco as model plants revealed that by inoculating Agrobacterium tumefaciens harboring these vectors, a large number of independently transformed roots could be induced from explants in a short period of time, and subsequent establishment of root culture lines was possible. A model experiment that focused on the sterol biosynthetic pathway in Arabidopsis validated the utility of pHR-RNAi vectors for gene functional analysis in cultured roots. Use of our vector system may facilitate identification of regulatory or biosynthetic genes for the production of valuable secondary metabolites in plant roots.
- 2008-09-01
著者
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Yoshida Shigeo
Plant Science Center, RIKEN
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Suzuki Hideyuki
Kazusa DNA Research Institute
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SEKI Hikaru
Kihara Institute for Biological Research, Yokohama City University
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OHYAMA Kiyoshi
RIKEN, Plant Science Center
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NISHIZAWA Tomoko
Kihara Institute for Biological Research, Yokohama City University
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YOSHIDA Shigeo
RIKEN, Plant Science Center
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MURANAKA Toshiya
Kihara Institute for Biological Research, Yokohama City University
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Sekizaki Haruo
Plant Science Center Riken
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Ohyama Kiyoshi
Plant Science Center Riken
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Ohyama Kiyoshi
Riken Kanagawa Jpn
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Ohyama K
Kyoto Univ. Kyoto Jpn
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Ohyama Kanji
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:laboratory Of Plant
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Yoshida Shigeo
Riken
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Yoshida Shigeo
Plant Science Center Riken
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Nishizawa Tomoko
Kihara Institute For Biological Research Yokohama City University
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Muranaka Toshiya
Kihara Institute For Biological Research Yokohama City University
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Seki Hikaru
Plant Sci. Center Riken
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Ohyama Kanji
Institute Of Bioresources And Biotechnology Ishikawa Prefectural University
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Shinshi Hideaki
Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.
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