ARTRA : a new database of the Arabidopsis transcriptome and gene-specific sequences for microarray probes and RNAi triggers
スポンサーリンク
概要
- 論文の詳細を見る
A new Arabidopsis transcriptome and microarray (ARTRA) database has been developed that contains transcriptome sequences and gene-specific sequences for a DNA microarray and RNAi knockdown triggers. Probes for the microarray were developed using sequences from the ARTRA database are characterized by long gene-specific fragments and spotting as a single sense strand. As a result, the gene-specificity and sensitivity of this microarray were both high, and therefore the ratio of gene expression from the present array was highly correlated with that from the Affymetrix GeneChip (R2=0.95). Moreover, AGAMOUS gene expression was reduced to about 10% of wild-type in T1 plants transformed with a vector containing an inverted repeat of the gene-specific fragment of AGAMOUS. Flowers of the T1 plants lacked pistils and stamens, which is the phenotype of known AGAMOUS knockout plants. This result showed that gene knockdown is possible in transgenic plants with the inverted repeat structure using a gene-specific fragment. RNAi knockdown is important as an approach for genes without T-DNA tag lines. Access to the ARTRA database is available to any researcher on request from the web site of http://artra.kazusa.or.jp/.
- 日本植物細胞分子生物学会の論文
- 2009-03-25
著者
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KATO Ikunoshin
Biotechnology Research Laboratories, Takara Bio Inc.
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Suzuki Hideyuki
Kazusa DNA Research Institute
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Sasaki Kanako
Research Institute For Sustainable Humanosphere Kyoto University
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SHIBATA Daisuke
Kazusa DNA Research Institute
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Asada Kiyozo
Biotechnology Research Laboratories, Takara Bio Inc.
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Sakurai Nozomu
Kazusa DNA Research Institute
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Suzuki Kaoru
Institute For Biological Resources And Functions Aist
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Noda Hideyuki
Dragon Genomics Center Takara Bio Inc.
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Ohba Toshiharu
Dragon Genomics Center Takara Bio Inc.
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Asada Kiyozo
Biotechnology Res. Laboratories Takara Bio Inc.
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Asada Kiyozo
Biotechnology Research Laboratories Takara Bio Inc.
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Shinshi H
Kazusa Dna Research Institute
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SUZUKI Kensuke
Dragon Genomics Center, Takara Bio Inc.
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OURA Tomonori
Dragon Genomics Center, Takara Bio Inc.
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ANDO Tatsuya
Dragon Genomics Center, Takara Bio Inc.
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Shibata Daisuke
Graduate School Of Life Sciences Tohoku University
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Seki Hikaru
Plant Sci. Center Riken
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Shimada Norimoto
Department Of Applied Biological Sciences Nihon University
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Sakakibara Norikazu
Institute For Sustainable Humanosphere Kyoto University
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Kato Ikunoshin
Biotechnology Research Laboratories Takara Bio Inc.
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Oura Tomonori
Dragon Genomics Center Takara Bio Inc.
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Ando Tatsuya
Dragon Genomics Center Takara Bio Inc.
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Shibata D
Graduate School Of Life Sciences Tohoku University
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Saito Kazuki
Chiba Univ. Chiba Jpn
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Shimada Norimoto
Department Of Life Science Aichi University Of Education
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Shibata Daisuke
Kazusa Dna Res. Inst. Chiba Jpn
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Shibata Daisuke
Kazusa Dna Res. Inst.
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Shibata Daisuke
Mitsui Plant Biotech. Res. Inst.
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Shinshi Hideaki
Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.
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Saito Kazuki
Dragon Genomics Center Takara Bio Inc.
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Suzuki Kaoru
Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.
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