Substrate-enantiomer selectivity of matairesinol O-methyltransferases
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概要
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Previously we reported a cDNA encoding an O-methyltransferase (OMT) responsible for the O-methylation of matairesinol to afford arctigenin in Carthamus tinctorius. However, the regioselectivity and stereoselectivity of its reaction are not yet well understood. In this paper, we report the characterization and comparison of three matairesinol OMTs (MROMTs) encoded by cDNAs isolated from C. tinctorius (CtMROMT), Anthriscus sylvestris (AsMROMT), and Forsythia koreana (FkMROMT). Although they shared matairesinol as a substrate, each recombinant MROMT showed different catalytic behavior. AsMROMT and CtMROMT methylated matairesinol's hydroxyl group at the C4′ position giving rise to arctigenin, while FkMROMT methylated the C4 position hydroxyl group giving rise to isoarctigenin. Analysis of the enantiomeric composition of products from racemic matairesinol showed all OMT reactions to be highly selective in terms of the substrate enantiomers and only use the (−)-enantiomer as the substrate.
- 日本植物細胞分子生物学会の論文
著者
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Suzuki Hideyuki
Kazusa DNA Research Institute
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Yamamura Masaomi
Research Institute For Sustainable Humanosphere Kyoto University
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Umezawa Toshiaki
Research Institute For Sustainable Humanosphere Kyoto University
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Shibata Daisuke
Kazusa Dna Res. Inst.
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HATTORI Takefumi
Research Institute for Sustainable Humanosphere, Kyoto University
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Ragamustari Safendrri
Research Institute for Sustainable Humanosphere, Kyoto University
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Ono Eiichiro
Research Department, Suntory Global Innovation Center Ltd.
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Ono Eiichiro
Research Institute, Suntory Global Innovation Center Ltd.
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Suzuki Shiro
Kazusa DNA Research Institute
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