A batch processing protocol for construction of expression vector plasmids from a cDNA collection and Agrobacterium-mediated transformation of suspension-cultured cells of Arabidopsis
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概要
- 論文の詳細を見る
In order to accelerate functional analysis of large transcription factor families, development of a simple and easy protocol to generate transgenic cells of Arabidopsis overexpressing the transgenes using collections of cDNAs for coding sequences (CDSs) of the family genes is promising. We conceived a batch processing protocol for construction of plant expression vector plasmids and Agrobacterium-mediated transformation of suspension-cultured cells using a Gateway system-compatible cDNA collection and demonstrated its feasibility using a cDNA collection of the DOF family. The present results suggested that the batch processing from the LR reaction to the transformation of Agrobacterium cells was properly performed. The specific overexpression of a single DOF transgene was observed in at least 6 of 8 lines, and a transgenic callus obviously overexpressing multiple DOF transgenes was not obtained. These results show the potential utility of the present protocol to gain a clue for detailed functional analyses of Arabidopsis genes.
- 日本植物細胞分子生物学会の論文
- 2009-09-25
著者
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Suzuki Hideyuki
Kazusa DNA Research Institute
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Sasaki Kanako
Research Institute For Sustainable Humanosphere Kyoto University
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Suzuki Kaoru
Institute For Biological Resources And Functions Aist
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Shinshi H
Kazusa Dna Research Institute
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NAITO Yuki
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science
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TSUJIMOTO INUI
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science
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NAKANO Toshitsugu
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science
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SHINSHI Hideaki
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science
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Nakatsubo Tomoyuki
Research Institute For Sustainable Humanosphere Kyoto University
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Seki Hikaru
Plant Sci. Center Riken
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Naito Yuki
Institute For Biological Resources And Functions Aist
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Nakano Toshitsugu
Institute For Biological Resources And Functions Aist
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Tsujimoto Inui
Institute For Biological Resources And Functions Aist
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Saito Kazuki
Chiba Univ. Chiba Jpn
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Shinshi Hideaki
Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.
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Suzuki Kaoru
Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.
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Tsujimoto-Inui Yayoi
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST)
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