Identification of Armillaria nabsnona in Gastrodia Tubers(Pharmacognosy)

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概要

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• The symbiosis between Armillaria species and an achlorophylous orchid Gastrodia elata BLUME has been reported. The main species described as a symbiont is Armillaria mellea (V_<AHL>: FR.) KUMMER, known widely as a primary root rot pathogen. Samples collected from the rhizomorphs attached to the tuber of G. elata were separated and analyzed. Molecular analysis based on sequencing of the intergenic spacer 1 (IGS-1) and internal transcribed spacer (ITS) regions of the ribosomal DNA (rDNA) was performed, coupled with restriction fragment length polymorphism (RFLP) of the IGS-1 region. Cultural morphology and features of basidiomata were also used to characterize the isolates. Phylogenetic analysis and morphological data strongly suggested that the fungus present in the tubers of G. elata is Armillaria nabsnona. This is the first report of occurrence of this Armillaria species in association with G. elata.

• 公益社団法人日本薬学会の論文
• 2008-07-01

著者

• Kojoma Mareshige

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Sekizaki Haruo

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Asazu Sandra

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Sawa Satoko

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Sawa Satoko

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Sekizaki Haruo

  Plant Science Center Riken

• Asazu Sandra

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• KUNINAGA Shiro

  School of Dentistry, Health Sciences University of Hokkaido

• YAMAMOTO Mizuho

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• YOKOSAWA Ryozo

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• YOSHIDA Naotoshi

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Yoshida Naotoshi

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Seki Hikaru

  Plant Sci. Center Riken

• Kojoma Mareshige

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Yokosawa Ryozo

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Kuninaga Shiro

  School Of Dentistry Health Sciences University Of Hokkaido

• Sekizaki Haruo

  Faculty Of Pharmaceutical Sciences Health Science University Of Hokkaido

• Yamamoto Mizuho

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Shinshi Hideaki

  Plant Mol. Biol. Lab. Nati. Inst. Biosci. Human Tech.

• Sekizaki Haruo

  Faculty of Pharmaceutical Science, Higashi Nippon Gakuen University

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