Trypsin-Catalyzed Synthesis of Dipeptide Containing α-Aminoisobutylic Acid Using p- and m-(Amidinomethyl)phenyl Esters as Acyl Donor

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概要

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• Two series of inverse substrates, p- and m-(amidinomethyl)phenyl esters derived from N-(tert-butyloxycarbonyl)amino acid, were prepared as acyl donor components for enzymatic peptide synthesis. They were found to be readily coupled with an acyl acceptor such as L-alanine p-nitroanilide to produce dipeptide. An α-amino-isobutyric acid containing dipeptide was especially obtained in satisfactory yield. Streptomyces griseus trypsin was a more efficient catalyst than the bovine trypsin. The optimum condition for the coupling reaction was studied by changing the organic solvent, pH, and acyl acceptor concentration. It was found that the enzymatic hydrolysis of the resulting product was negligible.

• 2008-05-01

著者

• Kojoma Mareshige

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Toyota Eiko

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Sekizaki Haruo

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Toyota E

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Toyota Eiko

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Itoh Kunihiko

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Kojoma Mareshige

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Sekizaki H

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Sekizaki Haruo

  Faculty Of Pharmaceutical Sciences Health Science University Of Hokkaido

• TANIZAWA Kazutaka

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• SHIBUYA Akiyoshi

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Tanizawa K

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Shibuya Akiyoshi

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Sekizaki Haruo

  Faculty of Pharmaceutical Science, Higashi Nippon Gakuen University

• Toyota Eiko

  Faculty of Pharmaceutical Science, Higashi Nippon Gakuen University

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