Enzymatic Peptide Synthesis with p-Guanidinophenyl and p-(Guanidinomethyl)phenyl Esters as Acyl Donors
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概要
- 論文の詳細を見る
Two series of "inverse substrates", N-Boc-amino acid p-guanidinophenyl and p-(guanidinomethyl)phenyl esters, were prepared as acyl donor components for enzymatic peptide synthesis. The kinetic behavior of these esters toward bovine and Streptomyces griseus (SG) trypsin was analyzed. The spatial requirement of the active site of these enzymes for catalytic efficiency is discussed based on the steric characteristics of the substrates. These substrates were found to couple readily with amino acid p-nitroanilides to produce peptides. SG trypsin was the most efficient catalyst among the enzymes tested (bovine, porcine, and SG trypsin).
- 1998-05-15
著者
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Toyota Eiko
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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Sekizaki Haruo
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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Toyota E
Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Toyota Eiko
Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Itoh Kunihiko
Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Sekizaki H
Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Sekizaki Haruo
Faculty Of Pharmaceutical Sciences Health Science University Of Hokkaido
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TANIZAWA Kazutaka
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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ITHO Kunihiko
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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Tanizawa K
Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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Toyoda Eiko
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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Sekizaki Haruo
Faculty of Pharmaceutical Science, Higashi Nippon Gakuen University
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