Application of Schiff Base Copper(II) and Iron(III) Chelates to Site-Specific Cleavage of a Trypsin

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概要

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• Amidine-containing Schiff base iron(III) and copper(II) chelates were prepared from α-amino acid, metal ion, and salicylaldehyde. These chelates behaved as specific inhibitors of trypsin, with K_i values in the range 10^<-5>-10^<-6> M. Selective cleavage of the trypsin backbone resulting from specific binding of the chelate to the trypsin active site was investigated. Cleavage was observed when trypsin was incubated with amidine-containing copper(II) or iron(III) chelate, H_2O_2,and ascorbate. Examination of the three-dimensional structure of trypsin suggests that cleavage occurred at a peptide bond within the Gly_<195>-Ala_<204> sequence.

• 1999-01-15

著者

• Toyota Eiko

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Sekizaki Haruo

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Toyota E

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Toyota Eiko

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Itoh Kunihiko

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Sekizaki H

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Sekizaki Haruo

  Faculty Of Pharmaceutical Sciences Health Science University Of Hokkaido

• Miyazaki Hiromitsu

  Faculty Of Pharmaceutical Sciences Health Science University Of Hokkaido

• TANIZAWA Kazutaka

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Tanizawa K

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• Sekizaki Haruo

  Faculty of Pharmaceutical Science, Higashi Nippon Gakuen University

• Toyota Eiko

  Faculty of Pharmaceutical Science, Higashi Nippon Gakuen University

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