Purification and Characterization of β-N-Acetylhexosaminidase from Trichoderma harzianum(Biological Chemistry)
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概要
- 論文の詳細を見る
β-N-Acetylhexosaminidase was produced by Trichoderma harzianum cultivated with chitin as the growth substrate. The enzyme was purified 13.2-fold to homogeneity by ultrafiltration and sequential chromatography on SP-Toyopearl and Sephacryl S-200. The molecular weight of the enzyme was estimated to be about 150,000 by gel filtration. The pH and temperature optima were 4.0-5.5 and 50℃, respectively. The enzyme hydrolyzed N-acetylchitooligosaccharides at the non-reducing ends to release GlcNAc monomer. The enzyme showed a strict substrate specificity to the sugar chains in complex carbohydrates, hydrolyzing only the linkage of GlcNAcβ-3Gal, but not hydrolyzing the other linkages such as GalNAcβ1-3Gal and GlcNAcβ1-2Man.
- 社団法人日本農芸化学会の論文
- 1991-11-23
著者
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KATO Ikunoshin
Biotechnology Research Laboratories, Takara Bio Inc.
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Koga Kenji
Biological Engineering Laboratory Asahi Industries Co. Ltd.
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Kato Ikunoshin
Biotechnology Research Laboratories Takara Bio Inc.
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Kato Ikunoshin
Biotechnology Research Laboratories Takara Shuzo Co. Ltd.
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Sano Mutsumi
Biotechnology Research Laboratories Takara Shuzo Co. Ltd.
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IWAMOTO Yasushi
Biological Engineering Laboratory, Asahi Industries Co., Ltd.
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SAKAMOTO Hiroshi
Biological Engineering Laboratory, Asahi Industries Co., Ltd.
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HATANO Kohta
Biological Engineering Laboratory, Asahi Industries Co., Ltd.
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Hatano Kohta
Biological Engineering Laboratory Asahi Industries Co. Ltd.
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Iwamoto Yasushi
Biological Engineering Laboratory Asahi Industries Co. Ltd.
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Sakamoto Hiroshi
Biological Engineering Laboratory Asahi Industries Co. Ltd.
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