Production of New Restriction Endonuclease VpaK11BI Recognizing Sequence 5'-GGWCC-3' in a Haemolysin-less Mutant of Vibrio parahaemolyticus
スポンサーリンク
概要
- 論文の詳細を見る
High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence of 5'-GGWCC-3' and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale.
- 社団法人日本農芸化学会の論文
- 1997-12-23
著者
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KATO Ikunoshin
Biotechnology Research Laboratories, Takara Bio Inc.
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ISHINO Yoshizumi
Biotechnology Research Laboratories, Takara Shuzo Co., Ltd.
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Kita Akihiko
Biotechnology Research Laboratories Takara Shuzo Co. Ltd.
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Kato Ikunoshin
Biotechnology Research Laboratories Takara Bio Inc.
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Kato Ikunoshin
Biotechnology Research Laboratories Takara Shuzo Co. Ltd.
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Miyahara M
Faculty Of Environmental And Symbiotic Sciences Prefectural University Of Kumamoto
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MIYAHARA Michiko
National Institute of Health Sciences
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Ishiwata N
National Institute Of Health Sciences
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Ueno H
Niigata Univ. Niigata‐shi Jpn
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UENO Harumi
Biotechnology Research Laboratories, Takara Shuzo Co., Ltd.
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ISHIWATA Naoko
National Institute of Health Sciences
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MISE Katsutoshi
National Institute of Health Sciences
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Ueno H
Nagaoka National College Of Technology
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Ishino Yoshizumi
Biotechnology Research Laboratories Takara Shuzo Co. Ltd.
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Ishino Yoshizumi
Biotechnology Research Laboratories Takara Shuzo Co. Ltd
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Miyahara M
Division Of Microbiology National Institute Of Health Sciences
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Ueno Harumi
Biotechnology Research Laboratories Takara Shuzo Co. Ltd.
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Miyahara Michiko
National Inst. Health Sci. Tokyo Jpn
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