Determination of o-Tyrosine Production in Aqueous Solutions of Phenylalanine Irradiated with Gamma Ray, Using High Performance Liquid Chromatography with Automated Pre-column Derivatization and LASER Fluorometric Detection

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概要

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• Tyrosine isomers produced by gamma radiation of aqueous phenylalanine solutions at mid dose levels (1-10kGy) were examined to obtain basic information for irradiated food detection using a new high performance liquid chromatography (HPLC) analytical procedure. The procedure was established using an automated pre-column derivatization with 4-fluoro-7-nitro 2,1,3-benzoxadiazole (NBD-F) followed by reverse phase HPLC and LASER fluorometric detection. The limit of detection (LOD) was 0.06ng on-column and the linear range for calibration was 0.06 to 50ng for the tyrosine derivatives. The relative standard deviation was 10% to 12%. The amounts of the tyrosine isomers increased with levels of irradiation. Irradiation at low temperature with reduced oxygen decreased the isomer yields. In the pH range of 5 to 7,the amount of product was not changed significantly by pH, outside this range, the pH did have an effect on product generation. At constant dose levels the yields of tyrosine isomers initially increased with phenylalanine concentration, although, with further increases in phenylalanine a reduction in the absolute amounts was observed. Dose rates varying from 0.5kGy/h to 10kGy/h had no significant effect on tyrosine isomer formation if a total of 10kGy was used in each case. In addition, demonstrating the usefulness of this new analytical technique for o-tyrosine determination, these studies suggest that the presence of o-tyrosine is another parameter indicative of gamma irradiation.

• 公益社団法人日本薬学会の論文
• 2000-06-01

著者

• TOYODA Masatake

  National Institute of Health Sciences

• SAITO Akiko

  Kitasato University, Sch. of Pharmaceutical Sciences

• Saito Yukio

  National Institute Of Health Sciences

• Saito Akiko

  School Of Allid Health Sciences Kitasato University

• Toyoda M

  Jissen Women's Univ.

• Saito Akiko

  Kitasato University Sch. Of Pharmaceutical Sciences

• Toyoda Masatake

  Faculty Of Human Life Sciences Jissen Women's University

• Saito Y

  National Institute Of Health Sciences

• Miyahara M

  Faculty Of Environmental And Symbiotic Sciences Prefectural University Of Kumamoto

• Izumi Keiko

  Jissen Women's College

• Miyahara Makoto

  National Institute of Health Sciences

• Kamimura Tomomi

  School of Allid Health Sciences, Kitasato University

• Nagasawa Taeko

  School of Allid Health Sciences, Kitasato University

• Ito Hitoshi

  The Japan Atomic Energy Research Institute Takasaki Radiation Establishment

• Nagasawa T

  Kitasato Univ. Kanagawa Jpn

• Kamimura Tomomi

  School Of Allid Health Sciences Kitasato University

• Takeda Mitsuharu

  College Of Bioresource Sciences Nihon University

• Kamimura Tomomi

  Kitasato University

• Nagasawa Taeko

  Kitasato University

• Ito Hitoshi

  Japan Atomic Energy Research Institute Takasaki Establishment

• Kariya Mari

  Kitasato University

• Kitamura Mayumi

  Jissen Women's College

• Kitamura Moritsugu

  Ishikawa Prefectural Institute Of Public Health And Environmental Science

• Miyahara M

  Division Of Microbiology National Institute Of Health Sciences

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